PDI Antibody / P4HB (RQ6356)

Immunofluorescent staining of FFPE human T-47D cells with PDI antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

IHC staining of FFPE human renal clear cell carcinoma tissue with PDI antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lymphoma tissue with PDI antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung cancer tissue with PDI antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liver cancer tissue with PDI antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE mouse spleen tissue with PDI antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat spleen tissue with PDI antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of human 1) HeLa, 2) PANC-1, 3) MCF7, 4) COLO-320, 5) HEK293, 6) HepG2, 7) monkey lung, 8) monkey liver, 9) rat liver, 10) mouse liver and 11) mouse NIH-3T3 lysate with PDI antibody. Predicted molecular weight ~57 kDa.

Flow cytometry testing of human U937 cells with PDI antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PDI antibody.