PDGFR Alpha Antibody / Receptor Tyrosine Kinase Antibody (RQ7956)

PDGFR Alpha Antibody IF. Immunofluorescent staining of FFPE human Caco-2 cells using PDGFR Alpha Antibody / Receptor Tyrosine Kinase Antibody demonstrates prominent membranous and cytoplasmic red fluorescence consistent with expression of PDGFRA. The staining pattern highlights cell borders and associated intracellular compartments, reflecting the localization of this cell-surface receptor tyrosine kinase. PDGFRA mediates platelet-derived growth factor signaling and regulates cellular proliferation, migration, differentiation, and survival through activation of downstream signaling pathways. Nuclei are counterstained with DAPI (blue). HIER was performed by steaming sections in pH 6 citrate buffer for 20 minutes prior to immunofluorescent staining. Red = PDGFR Alpha antibody; Blue = DAPI nuclear stain.

PDGFR Alpha Antibody Human Mouse Rat Brain WB. Western blot analysis of human 293T (lane 1), human U-251 (lane 2), rat brain (lane 3), rat C6 (lane 4), mouse brain (lane 5), and mouse NIH 3T3 (lane 6) lysates using PDGFR Alpha Antibody / Receptor Tyrosine Kinase Antibody demonstrates strong immunoreactive bands at approximately 180 kDa. PDGFRA is a cell-surface receptor tyrosine kinase that mediates platelet-derived growth factor signaling and regulates cellular proliferation, migration, differentiation, and survival. The observed molecular weight is consistent with mature glycosylated PDGFRA protein, which commonly migrates at substantially higher molecular weights than its predicted core protein size due to extensive post-translational glycosylation. The broad reactivity observed across human, rat, and mouse samples supports conserved expression of PDGFRA in neural and mesenchymal cell populations. Expected molecular weight: 120-195 kDa depending on glycosylation level.