PDGFR alpha Antibody / CD140a (R31979)

PDGFR Alpha Antibody Multi-Species WB. Western blot analysis of rat brain (lane 1), human HeLa (lane 2), and mouse NIH3T3 (lane 3) lysates using PDGFR Alpha Antibody / Receptor Tyrosine Kinase Antibody demonstrates strong immunoreactive bands at approximately 180 kDa. PDGFRA is a cell-surface receptor tyrosine kinase that mediates platelet-derived growth factor signaling and regulates cellular proliferation, migration, differentiation, and survival. The observed molecular weight is consistent with mature glycosylated PDGFRA, which commonly migrates at substantially higher apparent molecular weights than its predicted core protein size due to extensive post-translational glycosylation. Detection of comparable bands across rat, human, and mouse samples supports conserved expression of PDGFRA among multiple species. Expected and observed molecular weight: 120-195 kDa, depending on glycosylation level.

PDGFR Alpha Antibody Intestinal Cancer IHC. Immunohistochemistry staining of FFPE human intestinal cancer tissue using PDGFR Alpha Antibody / Receptor Tyrosine Kinase Antibody demonstrates cytoplasmic and membranous HRP-DAB brown staining within malignant gland-forming epithelial cells. The staining pattern is consistent with expression of PDGFRA, a receptor tyrosine kinase that mediates platelet-derived growth factor signaling and regulates cellular proliferation, migration, differentiation, and survival. PDGFRA has been implicated in tumor-associated growth factor signaling networks and contributes to cellular communication within the tumor microenvironment. The observed staining highlights PDGFRA expression in intestinal carcinoma-associated epithelial populations. HIER was performed by boiling tissue sections in pH 6, 10 mM citrate buffer for 20 minutes followed by cooling prior to immunostaining.

PDGFR Alpha Antibody Cancer Cell Panel WB. Western blot analysis of human HT1080 (lane 1), Caco-2 (lane 2), U-2 OS (lane 3), PC-3 (lane 4), and 293T (lane 5) cell lysates using PDGFR Alpha Antibody / Receptor Tyrosine Kinase Antibody demonstrates immunoreactive bands predominantly within the 180-195 kDa range, consistent with expression of glycosylated PDGFRA. Variation in apparent molecular weight and band intensity among cell lines is expected due to differences in receptor glycosylation, maturation state, and expression level. PDGFRA is a receptor tyrosine kinase that mediates platelet-derived growth factor signaling and regulates cellular proliferation, migration, differentiation, and survival. The observed banding patterns support endogenous PDGFRA expression across multiple human cancer-derived and immortalized cell lines. Expected and observed molecular weight: 120-195 kDa, depending on glycosylation level.