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Home >> Antibodies >> PDGF alpha Antibody / PDGFA / Platelet Derived Growth Factor A

PDGF alpha Antibody / PDGFA / Platelet Derived Growth Factor A (RQ6784)

  Catalog No Formulation Size Price (USD)  
Image RQ6784 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human SiHa cells with PDGF alpha antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HACAT, 2) rat PC-12 and 3) mouse lung tissue lysate with PDGF alpha antibody. Predicted molecular weight ~24 kDa (long form), ~22 kDa (short form).
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P04085
Applications Western blot : 1-2ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This PDGF alpha antibody is available for research use only.
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Description

Platelet-derived growth factor subunit A is a protein that in humans is encoded by the PDGFA gene. This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants.

Application Notes

Optimal dilution of the PDGF alpha antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids S87-T211) was used as the immunogen for the PDGF alpha antibody.

Storage

After reconstitution, the PDGF alpha antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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