PDE6D Antibody / Phosphodiesterase 6D Antibody (FY13531)

PDE6D Antibody by IF in human colon carcinoma. Immunofluorescence staining of a paraffin-embedded human colon carcinoma section with Phosphodiesterase 6D Antibody reveals red fluorescence within glandular epithelial structures, with blue DAPI nuclear counterstaining. Antigen retrieval was performed in EDTA buffer at pH 8.0. Sections were blocked with 10% goat serum and incubated with primary antibody at 5 ug/mL overnight at 4oC. Cy3-conjugated secondary antibody was applied at 1:500 for 30 minutes at 37oC. The image illustrates PDE6D-associated immunoreactivity in the epithelial tumor compartment.

PDE6D Antibody by IHC in human colon carcinoma. Immunohistochemistry of paraffin-embedded human colon carcinoma using Phosphodiesterase 6D Antibody demonstrates brown staining in gland-forming tumor epithelium, with hematoxylin highlighting cell nuclei. Sections underwent heat-mediated antigen retrieval in EDTA buffer at pH 8.0, followed by blocking with 10% goat serum. Primary antibody was applied at 2 ug/mL overnight at 4oC. Bound antibody was detected with a peroxidase-conjugated secondary reagent and DAB chromogen. The epithelial staining pattern provides tissue-level evidence of PDE6D immunoreactivity.

PDE6D Antibody by IHC in human testis. Immunohistochemistry of paraffin-embedded human testis with Phosphodiesterase 6D Antibody shows brown immunoreactivity in cells lining seminiferous tubules, against blue hematoxylin-counterstained nuclei. Heat-mediated epitope retrieval used EDTA buffer at pH 8.0. Sections were blocked with 10% goat serum and incubated with primary antibody at 2 ug/mL overnight at 4oC, followed by peroxidase-based detection with DAB. This tissue preparation demonstrates PDE6D-associated staining within the seminiferous epithelium.

PDE6D Antibody by IF in human testis. Immunofluorescence analysis of paraffin-embedded human testis using Phosphodiesterase 6D Antibody shows red fluorescence distributed among cells of the seminiferous tubules, with DAPI-stained nuclei appearing blue. EDTA buffer at pH 8.0 was used for antigen retrieval, followed by 10% goat serum blocking. Primary antibody was incubated at 5 ug/mL overnight at 4oC and detected with Cy3-conjugated secondary antibody at 1:500. The fluorescence signal illustrates the distribution of PDE6D immunoreactivity in testicular tissue.

PDE6D Antibody by WB in human and rodent samples. Western blot analysis using Phosphodiesterase 6D Antibody detects a principal band near 15-17 kDa in human Jurkat cells (lane 1), human placenta (lane 2), rat brain (lane 3), rat eye (lane 4), and mouse brain (lane 5). The observed migration is close to the calculated 17.4 kDa mass of PDE6D. Reduced lysates containing 30 ug protein per lane were separated by 12% SDS-PAGE and transferred to nitrocellulose. The primary antibody was incubated at 0.5 ug/mL overnight at 4oC, followed by HRP-conjugated secondary antibody and chemiluminescent detection.