PDE4C Antibody / 3',5'-cyclic-AMP phosphodiesterase 4C Antibody (FY13536)

PDE4C Antibody by FACS in K562 cells. Flow cytometric analysis using 3',5'-cyclic-AMP phosphodiesterase 4C Antibody shows a rightward fluorescence shift in stained K562 cells (blue histogram) relative to the rabbit IgG isotype control (green) and unstained cells (red). Cells were fixed in 4% paraformaldehyde and blocked with 10% goat serum. Primary antibody was applied at 1 ug per 1 x 10^6 cells for 30 minutes at 20oC, followed by DyLight 488-conjugated secondary antibody. The histogram documents antibody-associated fluorescence under the reported staining conditions.

PDE4C Antibody by WB in human and rodent lysates. Western blot analysis using 3',5'-cyclic-AMP phosphodiesterase 4C Antibody detects a band near 80 kDa in HepG2 (lane 1), K562 (lane 2), Caco-2 (lane 3), SH-SY5Y (lane 4), rat lung (lane 5), and mouse lung (lane 6). This migration agrees with the reported approximately 80 kDa molecular weight of PDE4C. Reduced lysates containing 30 ug protein per lane were separated by 10% SDS-PAGE and transferred to nitrocellulose. Membranes were incubated with primary antibody at 0.5 ug/mL overnight at 4oC and developed with an HRP-conjugated secondary antibody and chemiluminescent detection.