PDCL3 Antibody / Phosducin-like protein 3 Antibody (FY13545)

PDCL3 Antibody by IF in rat bladder tissue. Immunofluorescence analysis of paraffin-embedded rat bladder using Phosducin-like protein 3 Antibody shows strong red fluorescence within epithelial structures, with blue DAPI-stained nuclei outlining the tissue architecture. The merged image highlights PDCL3-associated staining around the epithelial cell layers and relatively less signal in surrounding tissue. Sections were subjected to heat-mediated antigen retrieval, blocking with 10% goat serum and overnight primary antibody incubation at 4oC. A fluorescent anti-rabbit secondary antibody was used to visualize the target-associated signal.

PDCL3 Antibody by IHC in mouse bladder tissue. Immunohistochemistry using Phosducin-like protein 3 Antibody demonstrates brown staining concentrated along the epithelial surfaces of folded glandular structures, with blue hematoxylin-stained nuclei visible in adjacent cells. Paraffin-embedded sections were subjected to EDTA-based heat-mediated antigen retrieval at pH 8.0, followed by blocking with 10% goat serum. Sections were incubated with 2 ug/mL primary antibody overnight at 4oC and developed with an HRP-conjugated secondary antibody and DAB. This view provides additional anatomical context for the observed PDCL3-associated signal.

PDCL3 Antibody by IF in cultured cells with alpha-tubulin counterstaining. Immunofluorescence analysis using Phosducin-like protein 3 Antibody demonstrates green PDCL3-associated fluorescence in cultured cells, together with red alpha-tubulin staining. The merged image shows green nuclear and perinuclear regions against a red cytoskeletal network, allowing comparison of the two cellular signals. Cells were blocked with goat serum and incubated overnight at 4oC with the target and alpha-tubulin primary antibodies. Fluorescent anti-rabbit and anti-mouse secondary antibodies were applied to visualize the corresponding targets.

PDCL3 Antibody by IP in MCF-7 cell lysate. Immunoprecipitation and Western blot analysis using Phosducin-like protein 3 Antibody demonstrates enrichment of a band near 34 kDa in the antibody immunoprecipitate compared with the control IgG condition. The input MCF-7 lysate contains a corresponding band at the expected PDCL3 position. The blot also shows an approximately 50 kDa IgG heavy-chain band in immunoprecipitated material, distinct from the target-associated signal. These data support recovery of PDCL3-associated protein from MCF-7 lysate under the tested immunoprecipitation conditions.

PDCL3 Antibody by WB in human cell lysates. Western blot analysis using Phosducin-like protein 3 Antibody detects a principal band near 34 kDa, consistent with the expected PDCL3 molecular weight. Lanes 1-4 contain A2780, COLO 320, MCF-7 and A549 whole-cell lysates, respectively. Reduced samples containing 30 ug protein per lane were separated by 12% SDS-PAGE and transferred to nitrocellulose. The primary antibody was incubated at 0.5 ug/mL overnight at 4oC, followed by HRP-conjugated secondary antibody and chemiluminescent detection. A comparably migrating PDCL3-associated band is visible in all four human cell lines.

PDCL3 Antibody by IHC in rat bladder tissue. Immunohistochemistry using Phosducin-like protein 3 Antibody shows brown staining along the epithelial lining of glandular structures, with hematoxylin counterstaining of surrounding nuclei. Paraffin-embedded sections underwent heat-mediated antigen retrieval in EDTA buffer, pH 8.0, and blocking with 10% goat serum. The primary antibody was applied at 2 ug/mL overnight at 4oC, followed by an HRP-conjugated anti-rabbit secondary antibody and DAB chromogen. The image illustrates PDCL3-associated staining in an organized epithelial compartment.

PDCL3 Antibody by IF in mouse bladder tissue. Immunofluorescence analysis using Phosducin-like protein 3 Antibody demonstrates red fluorescence in a folded epithelial structure with blue DAPI nuclear counterstaining. The signal follows the epithelial compartment and contrasts with adjacent regions showing comparatively little red fluorescence. Paraffin-embedded tissue underwent heat-mediated antigen retrieval and blocking before overnight incubation with primary antibody at 4oC. The target-associated fluorescence was detected with a labeled anti-rabbit secondary antibody. This image provides an additional view of PDCL3-associated staining within the tissue architecture.

PDCL3 Antibody by FACS in A2780 cells. Flow cytometry analysis using Phosducin-like protein 3 Antibody demonstrates a blue antibody-stained A2780 cell population shifted to higher fluorescence relative to the green isotype-control and red unstained distributions. Cells were fixed, permeabilized and blocked with normal goat serum before incubation with 1 ug primary antibody per 1 x 10^6 cells for 30 minutes at 20oC. Fluorescent anti-rabbit secondary antibody was applied for 30 minutes. The histogram demonstrates PDCL3-associated intracellular staining relative to the two controls.