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Home >> Antibodies >> PDCD1 Antibody / PD-1 / PD1

PDCD1 Antibody / PD-1 / PD1 [clone PDCD1/2720] (V7574)

  Catalog No Formulation Size Price (USD)  
Image V7574-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7574-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7574SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7574IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated
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IHC staining of FFPE human tonsil with PDCD1 antibody (clone PDCD1/2720). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using PDCD1 antibody (clone PDCD1/2720). These results demonstrate the foremost specificity of the PDCD1/2720 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free PDCD1 antibody (clone PDCD1/2720) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name PDCD1/2720
Purity Protein G affinity chromatography
UniProt Q15116
Localization Cell surface, cytoplasmic
Applications ELISA (order BSA/sodium azide-free format for coating) :
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This PDCD1 antibody is available for research use only.
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Description

PDCD-1 (programmed cell death-1 protein), also designated CD279, is a type I transmembrane receptor and a member of the immunoglobin gene superfamily. It is expressed on activated T-cells, B-cells, and myeloid cells. Anti-PDCD-1 is a marker of angioimmunoblastic lymphoma and suggests a unique cell of origin for this neoplasm. Unlike CD10 and BCL6, PDCD-1 is expressed by few B-cells, so anti-PDCD-1 may be a more specific and useful diagnostic marker in angioimmunoblastic lymphoma. In addition, PDCD-1 expression provides evidence that angioimmunoblastic lymphoma is a neoplasm derived from germinal center-associated T-cells.

Application Notes

Optimal dilution of the PDCD1 antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Human full-length recombinant PDCD1 protein was used as the immunogen for the PDCD1 antibody.

Storage

Store the PDCD1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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