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Home >> Antibodies >> Pdcd1 Antibody / Pd1

Pdcd1 Antibody / Pd1 (RQ7412)

  Catalog No Formulation Size Price (USD)  
Image RQ7412 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE rat thymus tissue with Pdcd1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse thymus tissue with Pdcd1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of mouse 1) EL-4, 2) spleen, 3) thymus, 4) RAW264.7 and 5) Ana-1 cell lysate with Pdcd1 antibody. Predicted molecular weight: 32-55 kDa depending on glycosylation level.
Availability 1-3 business days
Species Reactivity Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q02242
Localization Cell surface & cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This Pdcd1 antibody is available for research use only.
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Description

PDCD1 (Programmed cell death 1), also called PD1, encodes a cell surface receptor that is a member of the B7 superfamily involved in immunomodulation. This gene is mapped to 2q37.3. PDCD1 acts as an inhibitory molecule on T cells after interacting with its ligands PDL1 and PDL2. The PDCD1 gene contains 5 exons. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. Using flow cytometric analysis, it has been found that expression of PDCD1 was upregulated on CD16-positive and CD16-negative monocytes, but not on dendritic cells, in viremic HIV-positive patients, but not in highly active antiretroviral therapy (HAART)-treated HIV-positive patients. PDCD1 upregulation in monocytes was induced by microbial Toll-like receptor ligands and inflammatory cytokines.

Application Notes

Optimal dilution of the Pdcd1 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant mouse protein (amino acids A48-L288) was used as the immunogen for the Pdcd1 antibody.

Storage

After reconstitution, the Pdcd1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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