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Home >> Antibodies >> PD-L1 Antibody / B7-H1 / CD274

PD-L1 Antibody / B7-H1 / CD274 [clone RM320] (R20345)

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  Catalog No Formulation Size Price (USD)  
Image R20345-0.1ML Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ul 419
Microvalidated Recrabbitmono
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Western blot testing of human tumor cell line lysate with recombinant PD-L1 antibody at 1:1000. DU145 and LNCaP are prostate cancer cell lines with very minor PD-L1 expression. Predicted molecular weight ~34 kDa (unmodified), 45-70 kDa (glycosylated).
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Western blot testing of human tumor cell line lysate with recombinant PD-L1 antibody at 1:1000. DU145 and LNCaP are prostate cancer cell lines with very minor PD-L1 expression. Predicted molecular weight ~34 kDa (unmodified), 45-70 kDa (glycosylated).
IHC staining of FFPE human tonsil tissue with recombinant PD-L1 antibody at 1:250.
IHC staining of FFPE human tonsil tissue with recombinant PD-L1 antibody at 1:250.
IHC staining of FFPE human lung cancer tissue with recombinant PD-L1 antibody at 1:1000.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM320
Purity Protein A purified from animal origin-free supernatant
UniProt Q9EP73
Localization Cell surface, cytoplasmic
Applications Immunohistochemistry (FFPE) : 1:200-1:1000
Western blot : 1:500-1:1000
Immunocytochemistry : 1:200-1:500
Limitations This recombinant PD-L1 antibody is available for research use only.
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Description

This antibody reacts to human PD-L1 (CD274, B7-H1).

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant PD-L1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A peptide corresponding to the C-terminus of human PD-L1 was used as the immunogen for the recombinant PD-L1 antibody.

Storage

Store the recombinant PD-L1 antibody at -20oC.

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