PD-L1 Biotinylated Antibody / CD274 Detection Antibody [clone PDL1/2746] (V3955BTN)

PD-L1 Biotinylated Antibody Lung Carcinoma IHC. Immunohistochemistry analysis of PD-L1 (CD274) expression in FFPE human lung carcinoma using biotin-conjugated clone PDL1/2746. The antibody demonstrates membranous HRP-DAB brown staining in tumor epithelial cells with additional signal in tumor-associated immune cells, consistent with immune checkpoint activity within the tumor microenvironment. Biotin-streptavidin amplification enhances detection of both strong and lower-level PD-L1 expression, supporting improved visualization of heterogeneous staining across tumor regions. Heat-induced epitope retrieval was performed using pH 9 Tris-EDTA buffer.

PD-L1 Biotinylated Antibody Lung Carcinoma Tissue IHC. Immunohistochemistry analysis of PD-L1 (CD274) expression in FFPE human lung carcinoma using biotin-conjugated clone PDL1/2746. The antibody demonstrates strong membranous HRP-DAB brown staining in tumor cells with additional labeling of tumor-associated immune cells, consistent with checkpoint-related expression in the tumor microenvironment. Biotin-based detection supports enhanced signal intensity and improved sensitivity, enabling clear visualization of both high and moderate PD-L1 expression across tumor regions. Heat-induced epitope retrieval was performed using pH 9 Tris-EDTA buffer.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using PD-L1 antibody (clone PDL1/2746). These results demonstrate the foremost specificity of the PDL1/2746 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free PD-L1 antibody (clone PDL1/2746) as confirmation of integrity and purity.