PD-L1 Antibody / B7-H1 / CD274 [clone PDL1/2746] (V3955)

IHC testing of FFPE cervical carcinoma with PD-L1 antibody (clone PDL1/2746). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE breast carcinoma with PD-L1 antibody (clone PDL1/2746). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE lung SCC with PD-L1 antibody (clone PDL1/2746). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE basal cell carcinoma with PD-L1 antibody (clone PDL1/2746). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of human Jurkat cells with PD-L1 antibody (clone PDL1/2746) and a CF488 labeled secondary (green). Nuclei were counterstained with Reddot (red).

Western blot testing of mouse and human cell lysates with PD-L1 antibody (PDL1/2746). Expected molecular weight ~34 kDa (unmodified), 45-70 kDa (glycosylated).

Western blot testing of human cell lysates with PD-L1 antibody (PDL1/2746). Expected molecular weight ~34 kDa (unmodified), 45-70 kDa (glycosylated).

FACS testing of fixed and permeabilized human Jurkat cells with PD-L1 antibody; Blue=cells alone, Red=isotype control, Orange= PD-L1 antibody.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using PD-L1 antibody (clone PDL1/2746). These results demonstrate the foremost specificity of the PDL1/2746 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free PD-L1 antibody (clone PDL1/2746) as confirmation of integrity and purity.