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Home >> Antibodies >> PD-L1 Antibody / B7-H1 / CD274

PD-L1 Antibody / B7-H1 / CD274 [clone AC37] (F55054)

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Image F55054-0.1ML Antibody in 1X PBS with 0.05% BSA and 0.05% ProClin 300 0.1 ml 399
Microvalidated Recrabbitmono
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IHC staining of FFPE human tonsil tissue with PD-L1 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
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IHC staining of FFPE human tonsil tissue with PD-L1 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
IHC staining of FFPE human tonsil tissue with PD-L1 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
IHC staining of FFPE human esophageal squamous carcinoma tissue with PD-L1 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
IHC staining of FFPE human NCI-H226 cells (left) and PD-L1 transfected HEK293 cells (right) with PD-L1 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
Western blot testing of PD-L1 transfected human 293 cell lysate (left) and non-transfected 293 cell lysate (right). Predicted molecular weight ~34 kDa (unmodified), 45-70 kDa (glycosylated).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name AC37
Purity Protein A affinity chromatography
UniProt Q9NZQ7
Localization Cell surface, cytoplasmic
Applications Western blot : 1:500-1:1000
Immunohistochemistry (FFPE) : 1:100-1:400
Limitations This PD-L1 antibody is available for research use only.
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Description

As a ligand for the inhibitory receptor PDCD1/PD-1, PD-L1 modulates the activation threshold of T-cells and limits T-cell effector response. [UniProt]

Application Notes

Optimal dilution of the PD-L1 antibody should be determined by the researcher.

Storage

Aliquot and store the PD-L1 antibody at 2-8oC.

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