PD-L1 Antibody for FACS / CD274 Flow Cytometry Antibody [clone PDL1/2743] (V7823)

PD-L1 Antibody for FACS. Flow cytometry analysis of PD-L1 (CD274) expression in human Jurkat cells using mouse monoclonal clone PDL1/2743. The PD-L1 antibody (orange) demonstrates a clear right-shifted population compared to isotype control (red) and unstained cells (blue), indicating specific cell surface expression of CD274. The distinct separation between negative and positive populations supports accurate gating and reliable identification of PD-L1-expressing cells, consistent with high-specificity performance in flow cytometry assays.

Immunofluorescent staining of human Jurkat cells with PD-L1 antibody (clone PDL1/2743, green) and Reddot nuclear stain (red).

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using PD-L1 antibody (clone PDL1/2743). These results demonstrate the foremost specificity of the PDL1/2743 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free PD-L1 antibody (clone PDL1/2743) as confirmation of integrity and purity.