PCTK1 Antibody / PCTAIRE Kinase Antibody (RQ6042)

PCTK1 Antibody Human Cervical Cancer Cell IF. Immunofluorescent staining of SiHa cells using PCTK1 antibody demonstrated predominantly cytoplasmic fluorescence (red), consistent with the localization of this PCTAIRE family kinase in intracellular signaling and vesicle trafficking pathways. Cells were treated with enzyme antigen retrieval reagent for 15 minutes, blocked with 10% goat serum, and incubated with the primary antibody (5 ug/ml) overnight at 4°C. Cy3-conjugated goat anti-rabbit IgG was used as the secondary antibody, and nuclei were counterstained with DAPI (blue). This PCTK1 Antibody, also called a PCTAIRE Kinase Antibody, is useful for investigating cyclin-dependent kinase signaling, vesicle trafficking, neuronal biology, and reproductive biology.

PCTK1 Antibody Human Placenta IHC. Immunohistochemical staining of FFPE human placenta tissue using PCTK1 antibody demonstrated predominantly cytoplasmic staining of trophoblast cells, consistent with the expression of this atypical cyclin-dependent kinase in actively proliferating and differentiating tissues. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen. This PCTK1 Antibody, also called a PCTAIRE Kinase Antibody, is useful for investigating cyclin-dependent kinase signaling, vesicle trafficking, neuronal biology, and reproductive biology.

PCTK1 Antibody Human, Rat and Mouse WB. Western blot testing of human 293T (lane 1), MCF-7 (lane 2), SiHa (lane 3), A431 (lane 4), rat testis (lane 5), and mouse testis (lane 6) lysates using PCTK1 antibody detected a major band at approximately 65 kDa. Although the calculated molecular weight of CDK16/PCTK1 is approximately 56 kDa, slower migration is commonly observed, and the closely spaced doublet seen in several samples is consistent with different phosphorylation states or other post-translationally modified forms reported for this kinase. This PCTK1 Antibody, also called a PCTAIRE Kinase Antibody, is useful for investigating cyclin-dependent kinase signaling, vesicle trafficking, neuronal biology, and reproductive biology.