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Home >> Antibodies >> PCTK1 Antibody / PCTAIRE1 / CDK16

PCTK1 Antibody / PCTAIRE1 / CDK16 (RQ6042)

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  Catalog No Formulation Size Price (USD)  
Image RQ6042 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human lung cancer with PCTAIRE1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human lung cancer with PCTAIRE1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human Caco-2, 2) rat PC-12 and 3) mouse NIH 3T3 lysate with PCTAIRE1 antibody. Predicted molecular weight ~56 kDa.
Flow cytometry testing of human 293T cells with PCTK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PCTK1 antibody.
Flow cytometry testing of human U-2 OS cells with PCTK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PCTK1 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q00536
Localization Cytoplasmic, plasma membrane
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry : 1-2ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This PCTK1 antibody is available for research use only.
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Description

Cyclin-dependent kinase 16 is a protein that in humans is encoded by the CDK16 gene. This gene is mapped to Xp11.3. The protein encoded by this gene belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. It may play a role in signal transduction cascades in terminally differentiated cells; in exocytosis; and in transport of secretory cargo from the endoplasmic reticulum. This gene is thought to escape X inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.

Application Notes

Optimal dilution of the PCTK1 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids N392-H443) was used as the immunogen for the PCTK1 antibody.

Storage

After reconstitution, the PCTK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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