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Home >> Antibodies >> PCNA Antibody

PCNA Antibody [clone DO-16] (RQ5380)

  Catalog No Formulation Size Price (USD)  
Image RQ5380 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 429
Microvalidated Rab Mono Image
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Western blot testing of 1) human HeLa, 2) human HepG2, 3) human U937 and 4) mouse spleen lysate with PCNA antibody. Predicted molecular weight ~29 kDa, routinely observed at 29~36 kDa.
IHC staining of FFPE human colon tissue with PCNA antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Availability 1-2 weeks
Species Reactivity Human, Mouse
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name DO-16
Purity Affinity purified
UniProt P12004
Localization Nuclear
Applications Western blot : 1:3000-1:10000
Immunohistochemistry (FFPE) : 1:50-1:200
Limitations This PCNA antibody is available for research use only.
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Description

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. [RefSeq]

Application Notes

Optimal dilution of the PCNA antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to human PCNA was used as the immunogen for the PCNA antibody.

Storage

Store the PCNA antibody at -20oC.

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