- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
PCNA Antibody / Proliferating Cell Nuclear Antigen [clone PCNA/8696R] (V4827)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V4827-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
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V4827-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
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V4827SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Clone Name | PCNA/8696R |
| Purity | Protein A/G affinity |
| UniProt | P12004 |
| Localization | Nucleus, Cytoplasm |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT Western Blot : 2-4ug/ml |
| Limitations | This PCNA antibody is available for research use only. |
|
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PCNA antibody, also known as Proliferating Cell Nuclear Antigen antibody, recognizes a nuclear protein commonly referred to as Cyclin and DNA polymerase delta auxiliary protein. Proliferating Cell Nuclear Antigen is encoded by the PCNA gene located on chromosome 20p12 and is a highly conserved component of the DNA replication machinery. PCNA is predominantly localized to the nucleus, where it functions as a sliding clamp that encircles DNA and coordinates replication and repair processes. It is strongly expressed in proliferating cells, particularly during the S phase of the cell cycle, and is widely used as a marker of cellular proliferation.
Proliferating Cell Nuclear Antigen plays a central role in DNA replication by acting as a processivity factor for DNA polymerase delta and epsilon. By forming a homotrimeric ring structure, it tethers polymerases and other replication factors to DNA, enhancing synthesis efficiency. PCNA also participates in multiple DNA repair pathways, including base excision repair, nucleotide excision repair, and mismatch repair, by recruiting repair enzymes to sites of DNA damage. PCNA antibody is frequently used in studies examining cell cycle progression, DNA replication dynamics, and tumor growth.
Structurally, Proliferating Cell Nuclear Antigen forms a toroidal homotrimer that creates a central channel for double-stranded DNA. Post-translational modifications such as ubiquitination and phosphorylation regulate PCNA interactions with distinct repair or tolerance factors, influencing pathway selection during DNA damage responses. Through interactions with numerous PCNA-interacting protein motifs, the protein acts as a central hub integrating replication, chromatin remodeling, and checkpoint signaling pathways.
Elevated PCNA expression is associated with increased proliferative activity in a wide range of malignancies, including breast, colorectal, lung, and prostate cancers. Its strong nuclear staining in dividing tumor cells makes it a widely used biomarker in pathology for assessing proliferation index. Beyond oncology, PCNA is essential for normal tissue renewal and regeneration, reflecting its fundamental role in maintaining genomic stability during cell division.
PCNA antibody supports research into cell cycle regulation, DNA replication, and genomic maintenance. Recombinant monoclonal clone PCNA/8696R recognizes Proliferating Cell Nuclear Antigen and is suitable for detecting PCNA expression in relevant research applications.
Optimal dilution of the PCNA antibody should be determined by the researcher.
Recombinant full-length human PCNA protein was used as the immunogen for the PCNA antibody.
Aliquot the PCNA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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