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Home >> Antibodies >> PCNA Antibody / Proliferating Cell Nuclear Antigen

PCNA Antibody / Proliferating Cell Nuclear Antigen [clone PCNA/8633R] (V4804)

  Catalog No Formulation Size Price (USD)  
Image V4804-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4804-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4804SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human tonsil tissue with PCNA antibody (clone PCNA/8633R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free PCNA antibody (clone PCNA/8633R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name PCNA/8633R
Purity Protein A/G affinity
UniProt P12004
Localization Nucleus, Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This PCNA antibody is available for research use only.
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Description

Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This mAb is excellent for multiple applications.

Application Notes

Optimal dilution of the PCNA antibody should be determined by the researcher.

Immunogen

Recombinant full-length human PCNA protein was used as the immunogen for the PCNA antibody.

Storage

Aliquot the PCNA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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