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Home >> Antibodies >> PCNA Antibody

PCNA Antibody [clone PM441-1] (V7004)

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  Catalog No Formulation Size Price (USD)  
Image V7004-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7004-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7004SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7004IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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Formalin-fixed, paraffin-embedded human tonsil stained with PCNA antibody (clone PM441-1). FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
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Formalin-fixed, paraffin-embedded human tonsil stained with PCNA antibody (clone PM441-1). FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
Western blot analysis of HeLa cell lysate using PCNA antibody (clone PM441-1). Predicted molecular weight 29~36 kDa.
Flow cytometry testing of PFA-fixed human HeLa cells with PCNA antibody (clone PM441-1); Red=isotype control, Blue= PCNA antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name PM441-1
Purity Protein G affinity chromatography
UniProt P12004
Localization Nuclear with some cytoplasmic
Applications Flow cytometry : 1-2ug/10^6 cells
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT (1)
Prediluted IHC only format : incubate for 30 min at RT (2)
Limitations This PCNA antibody is available for research use only.
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Description

Proliferating cell nuclear antigen is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. [UniProt]

Application Notes

Titering of the PCNA antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant proliferating cell nuclear antigen was used as the immunogen for the PCNA antibody.

Storage

Store the PCNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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