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Home >> Antibodies >> PCNA Antibody

PCNA Antibody [clone PC5] (V7998)

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  Catalog No Formulation Size Price (USD)  
Image V7998-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7998-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7998SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human tonsil with PCNA antibody (clone PC5). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human tonsil with PCNA antibody (clone PC5). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon carcinoma with PCNA antibody (clone PC5). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human samples with PCNA antibody (clone PC5). Expected molecular weight: 29-36 kDa.
SDS-PAGE analysis of purified, BSA-free PCNA antibody (clone PC5) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name PC5
Purity Protein G affinity chromatography
UniProt P12004
Localization Predominantly nuclear, some cytoplasmic
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This PCNA antibody is available for research use only.
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Description

Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases.

Application Notes

Optimal dilution of the PCNA antibody should be determined by the researcher.

Immunogen

A rat PCNA/protein A fusion protein was used at the immunogen for this PCNA antibody.

Storage

Store the PCNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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