PCNA Antibody [clone PC10] (V2218)

Description

This antibody recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). PCNA is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA-cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This antibody is excellent for the detection of PCNA in multiple applications.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Rat PCNA / Protein A fusion protein was used as the immunogen for this antibody.

Storage

Store the PCNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Cyclin; Mutagen-sensitive 209 protein; PCNAR; Polymerase delta accessory protein, PCNA antibody