PCK2 Antibody | Mitochondrial Gluconeogenesis Enzyme

	Catalog No	Formulation	Size	Price (USD)
	RQ5647	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	449
Formulation

PCK2 Antibody Human Thyroid Cancer IHC. PCK2 was detected in a paraffin-embedded section of human thyroid cancer tissue following heat-mediated antigen retrieval in EDTA buffer, pH 8.0. The section was blocked with 10% goat serum and incubated overnight at 4Â°C with rabbit anti-PCK2 antibody at 2 ug/ml. An HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen were used for visualization. Strong cytoplasmic staining is visible in malignant thyroid epithelial cells, with comparatively limited signal in the surrounding stroma. PCK2 Antibody, a mitochondrial gluconeogenesis enzyme antibody, supports studies of metabolic reprogramming in thyroid cancer.

PCK2 Antibody Human Ovarian Cancer IHC. PCK2 was detected in a paraffin-embedded section of human ovarian cancer tissue following heat-mediated antigen retrieval in EDTA buffer, pH 8.0. The section was blocked with 10% goat serum and incubated overnight at 4Â°C with rabbit anti-PCK2 antibody at 2 ug/ml. An HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen were used for visualization. The staining demonstrates granular cytoplasmic PCK2 expression in ovarian cancer cells. PCK2 Antibody, a mitochondrial gluconeogenesis enzyme antibody, supports the examination of PCK2 distribution in cancer metabolism research.

PCK2 Antibody Human Pancreatic Cancer IHC. PCK2 was detected in a paraffin-embedded section of human pancreatic cancer tissue following heat-mediated antigen retrieval in EDTA buffer, pH 8.0. The section was blocked with 10% goat serum and incubated overnight at 4Â°C with rabbit anti-PCK2 antibody at 2 ug/ml. An HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen were used for visualization. The staining shows cytoplasmic PCK2 expression in pancreatic cancer cells, with variable signal intensity across the tissue. PCK2 Antibody, a mitochondrial gluconeogenesis enzyme antibody, supports research into metabolic enzyme expression in pancreatic cancer.

PCK2 Antibody Human Liver Cancer IHC. PCK2 was detected in a paraffin-embedded section of human liver cancer tissue following heat-mediated antigen retrieval in EDTA buffer, pH 8.0. The section was blocked with 10% goat serum and incubated overnight at 4Â°C with rabbit anti-PCK2 antibody at 2 ug/ml. An HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen were used for visualization. The staining reveals widespread cytoplasmic PCK2 expression in malignant cells, with variable intensity across the tumor. PCK2 Antibody, a mitochondrial gluconeogenesis enzyme antibody, supports studies of altered carbon metabolism in liver cancer.

PCK2 Antibody Human Lung Cancer IHC. PCK2 was detected in a paraffin-embedded section of human lung cancer tissue following heat-mediated antigen retrieval in EDTA buffer, pH 8.0. The section was blocked with 10% goat serum and incubated overnight at 4Â°C with rabbit anti-PCK2 antibody at 2 ug/ml. An HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen were used for visualization. The staining demonstrates moderate, predominantly cytoplasmic PCK2 expression in malignant lung epithelial cells. PCK2 Antibody, a mitochondrial gluconeogenesis enzyme antibody, supports research into metabolic adaptation and mitochondrial carbon metabolism in lung cancer.

PCK2 Antibody Human Stomach Cancer IHC. PCK2 was detected in a paraffin-embedded section of human stomach cancer tissue following heat-mediated antigen retrieval in EDTA buffer, pH 8.0. The section was blocked with 10% goat serum and incubated overnight at 4Â°C with rabbit anti-PCK2 antibody at 2 ug/ml. An HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen were used for visualization. The staining reveals widespread granular cytoplasmic PCK2 expression in malignant gastric cells. PCK2 Antibody, a mitochondrial gluconeogenesis enzyme antibody, supports studies of metabolic adaptation and mitochondrial carbon utilization in stomach cancer.

PCK2 Antibody Human Tonsil IHC. PCK2 was detected in a paraffin-embedded section of human tonsil tissue following heat-mediated antigen retrieval in EDTA buffer, pH 8.0. The section was blocked with 10% goat serum and incubated overnight at 4Â°C with rabbit anti-PCK2 antibody at 2 ug/ml. An HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen were used for visualization. The staining demonstrates variable cytoplasmic PCK2 expression among cells within the tonsillar tissue. PCK2 Antibody, a mitochondrial gluconeogenesis enzyme antibody, supports studies of metabolic enzyme distribution in human lymphoid tissue.

PCK2 Antibody Mouse Lung Airway IHC. PCK2 was detected in a paraffin-embedded section of mouse lung tissue following heat-mediated antigen retrieval in EDTA buffer, pH 8.0. The section was blocked with 10% goat serum and incubated overnight at 4Â°C with rabbit anti-PCK2 antibody at 2 ug/ml. An HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen were used for visualization. Strong cytoplasmic staining is present in airway epithelial cells, with weaker expression in the surrounding alveolar tissue. PCK2 Antibody, a mitochondrial gluconeogenesis enzyme antibody, supports comparative studies of pulmonary energy metabolism.

PCK2 Antibody Rat Lung Bronchiole IHC. PCK2 was detected in a paraffin-embedded section of rat lung tissue following heat-mediated antigen retrieval in EDTA buffer, pH 8.0. The section was blocked with 10% goat serum and incubated overnight at 4Â°C with rabbit anti-PCK2 antibody at 2 ug/ml. An HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen were used for visualization. Strong granular cytoplasmic staining is present in bronchiolar epithelial cells, with weaker signal in the surrounding alveolar tissue. PCK2 Antibody, a mitochondrial gluconeogenesis enzyme antibody, supports comparative studies of pulmonary energy metabolism.

PCK2 Antibody MCF7 Breast Cancer Cell IF. PCK2 was detected in formalin-fixed, paraffin-embedded human MCF7 breast cancer cells following heat-induced epitope retrieval by steaming in citrate buffer, pH 6.0, for 20 minutes. Immunofluorescent staining shows PCK2 in green, while nuclei were counterstained with DAPI and appear blue. The observed signal is predominantly cytoplasmic and perinuclear, consistent with the mitochondrial localization of PCK2. PCK2 Antibody, a mitochondrial gluconeogenesis enzyme antibody, supports examination of PCK2 expression and subcellular distribution in breast cancer metabolism research.

PCK2 Antibody HeLa Cell IF. PCK2 was detected in HeLa cells following enzyme-mediated antigen retrieval for 15 minutes. The cells were blocked with 10% goat serum and incubated overnight at 4Â°C with rabbit anti-PCK2 antibody at 5 ug/ml. A Cy3-conjugated goat anti-rabbit IgG secondary antibody was used at 1:500, and nuclei were counterstained with DAPI. PCK2 staining appears red and displays a punctate cytoplasmic pattern consistent with the mitochondrial distribution of the target protein, while nuclei appear blue. PCK2 Antibody, a mitochondrial gluconeogenesis enzyme antibody, supports cellular localization and metabolic pathway studies.

PCK2 Antibody Human and Monkey Cell and Tissue WB. Western blot analysis was performed under reducing conditions using 30 ug of lysate per lane. Lane 1: human T-47D cells; Lane 2: human U-2 OS cells; Lane 3: human A431 cells; Lane 4: human K562 cells; Lane 5: monkey COS-7 cells; Lane 6: monkey lung tissue. The membrane was incubated overnight at 4Â°C with rabbit anti-PCK2 antibody at 0.5 ug/ml, followed by an HRP-conjugated secondary antibody and ECL detection. A specific band was observed at approximately 71 kDa. PCK2 Antibody, a mitochondrial gluconeogenesis enzyme antibody, detects endogenous PCK2 across human and monkey samples.

PCK2 Antibody Rat and Mouse Tissue WB. Western blot analysis was performed under reducing conditions using 30 ug of tissue lysate per lane. Lane 1: rat kidney; Lane 2: rat liver; Lane 3: mouse lung; Lane 4: mouse spleen; Lane 5: mouse kidney; Lane 6: mouse liver. The membrane was incubated overnight at 4Â°C with rabbit anti-PCK2 antibody at 0.5 ug/ml, followed by an HRP-conjugated secondary antibody and ECL detection. A specific band was observed at approximately 71 kDa in each sample, with variable expression intensity. PCK2 Antibody, a mitochondrial gluconeogenesis enzyme antibody, detects endogenous PCK2 across rat and mouse tissues.

PCK2 Antibody MCF7 Breast Cancer Cell FACS. Intracellular flow cytometry analysis was performed using human MCF7 breast cancer cells fixed with 4% paraformaldehyde and permeabilized before staining. Cells were blocked with 10% normal goat serum and incubated with rabbit anti-PCK2 antibody at 1 ug per million cells for 30 minutes at 20Â°C, followed by a Fluoro488-conjugated goat anti-rabbit IgG secondary antibody. The blue trace represents PCK2 staining, the green trace represents the isotype control and the red trace represents unstained cells. The rightward shift demonstrates detectable intracellular PCK2 expression. PCK2 Antibody, a mitochondrial gluconeogenesis enzyme antibody, supports metabolic analysis by flow cytometry.

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PCK2 Antibody Human Thyroid Cancer IHC. PCK2 was detected in a paraffin-embedded section of human thyroid cancer tissue following heat-mediated antigen retrieval in EDTA buffer, pH 8.0. The section was blocked with 10% goat serum and incubated overnight at 4°C with rabbit anti-PCK2 antibody at 2 ug/ml. An HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen were used for visualization. Strong cytoplasmic staining is visible in malignant thyroid epithelial cells, with comparatively limited signal in the surrounding stroma. PCK2 Antibody, a mitochondrial gluconeogenesis enzyme antibody, supports studies of metabolic reprogramming in thyroid cancer.

PCK2 Antibody Human Ovarian Cancer IHC. PCK2 was detected in a paraffin-embedded section of human ovarian cancer tissue following heat-mediated antigen retrieval in EDTA buffer, pH 8.0. The section was blocked with 10% goat serum and incubated overnight at 4°C with rabbit anti-PCK2 antibody at 2 ug/ml. An HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen were used for visualization. The staining demonstrates granular cytoplasmic PCK2 expression in ovarian cancer cells. PCK2 Antibody, a mitochondrial gluconeogenesis enzyme antibody, supports the examination of PCK2 distribution in cancer metabolism research.

PCK2 Antibody Human Pancreatic Cancer IHC. PCK2 was detected in a paraffin-embedded section of human pancreatic cancer tissue following heat-mediated antigen retrieval in EDTA buffer, pH 8.0. The section was blocked with 10% goat serum and incubated overnight at 4°C with rabbit anti-PCK2 antibody at 2 ug/ml. An HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen were used for visualization. The staining shows cytoplasmic PCK2 expression in pancreatic cancer cells, with variable signal intensity across the tissue. PCK2 Antibody, a mitochondrial gluconeogenesis enzyme antibody, supports research into metabolic enzyme expression in pancreatic cancer.

PCK2 Antibody Human Liver Cancer IHC. PCK2 was detected in a paraffin-embedded section of human liver cancer tissue following heat-mediated antigen retrieval in EDTA buffer, pH 8.0. The section was blocked with 10% goat serum and incubated overnight at 4°C with rabbit anti-PCK2 antibody at 2 ug/ml. An HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen were used for visualization. The staining reveals widespread cytoplasmic PCK2 expression in malignant cells, with variable intensity across the tumor. PCK2 Antibody, a mitochondrial gluconeogenesis enzyme antibody, supports studies of altered carbon metabolism in liver cancer.

PCK2 Antibody Human Lung Cancer IHC. PCK2 was detected in a paraffin-embedded section of human lung cancer tissue following heat-mediated antigen retrieval in EDTA buffer, pH 8.0. The section was blocked with 10% goat serum and incubated overnight at 4°C with rabbit anti-PCK2 antibody at 2 ug/ml. An HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen were used for visualization. The staining demonstrates moderate, predominantly cytoplasmic PCK2 expression in malignant lung epithelial cells. PCK2 Antibody, a mitochondrial gluconeogenesis enzyme antibody, supports research into metabolic adaptation and mitochondrial carbon metabolism in lung cancer.

PCK2 Antibody Human Stomach Cancer IHC. PCK2 was detected in a paraffin-embedded section of human stomach cancer tissue following heat-mediated antigen retrieval in EDTA buffer, pH 8.0. The section was blocked with 10% goat serum and incubated overnight at 4°C with rabbit anti-PCK2 antibody at 2 ug/ml. An HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen were used for visualization. The staining reveals widespread granular cytoplasmic PCK2 expression in malignant gastric cells. PCK2 Antibody, a mitochondrial gluconeogenesis enzyme antibody, supports studies of metabolic adaptation and mitochondrial carbon utilization in stomach cancer.

PCK2 Antibody Human Tonsil IHC. PCK2 was detected in a paraffin-embedded section of human tonsil tissue following heat-mediated antigen retrieval in EDTA buffer, pH 8.0. The section was blocked with 10% goat serum and incubated overnight at 4°C with rabbit anti-PCK2 antibody at 2 ug/ml. An HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen were used for visualization. The staining demonstrates variable cytoplasmic PCK2 expression among cells within the tonsillar tissue. PCK2 Antibody, a mitochondrial gluconeogenesis enzyme antibody, supports studies of metabolic enzyme distribution in human lymphoid tissue.

PCK2 Antibody Mouse Lung Airway IHC. PCK2 was detected in a paraffin-embedded section of mouse lung tissue following heat-mediated antigen retrieval in EDTA buffer, pH 8.0. The section was blocked with 10% goat serum and incubated overnight at 4°C with rabbit anti-PCK2 antibody at 2 ug/ml. An HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen were used for visualization. Strong cytoplasmic staining is present in airway epithelial cells, with weaker expression in the surrounding alveolar tissue. PCK2 Antibody, a mitochondrial gluconeogenesis enzyme antibody, supports comparative studies of pulmonary energy metabolism.

PCK2 Antibody Rat Lung Bronchiole IHC. PCK2 was detected in a paraffin-embedded section of rat lung tissue following heat-mediated antigen retrieval in EDTA buffer, pH 8.0. The section was blocked with 10% goat serum and incubated overnight at 4°C with rabbit anti-PCK2 antibody at 2 ug/ml. An HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen were used for visualization. Strong granular cytoplasmic staining is present in bronchiolar epithelial cells, with weaker signal in the surrounding alveolar tissue. PCK2 Antibody, a mitochondrial gluconeogenesis enzyme antibody, supports comparative studies of pulmonary energy metabolism.

PCK2 Antibody MCF7 Breast Cancer Cell IF. PCK2 was detected in formalin-fixed, paraffin-embedded human MCF7 breast cancer cells following heat-induced epitope retrieval by steaming in citrate buffer, pH 6.0, for 20 minutes. Immunofluorescent staining shows PCK2 in green, while nuclei were counterstained with DAPI and appear blue. The observed signal is predominantly cytoplasmic and perinuclear, consistent with the mitochondrial localization of PCK2. PCK2 Antibody, a mitochondrial gluconeogenesis enzyme antibody, supports examination of PCK2 expression and subcellular distribution in breast cancer metabolism research.

PCK2 Antibody HeLa Cell IF. PCK2 was detected in HeLa cells following enzyme-mediated antigen retrieval for 15 minutes. The cells were blocked with 10% goat serum and incubated overnight at 4°C with rabbit anti-PCK2 antibody at 5 ug/ml. A Cy3-conjugated goat anti-rabbit IgG secondary antibody was used at 1:500, and nuclei were counterstained with DAPI. PCK2 staining appears red and displays a punctate cytoplasmic pattern consistent with the mitochondrial distribution of the target protein, while nuclei appear blue. PCK2 Antibody, a mitochondrial gluconeogenesis enzyme antibody, supports cellular localization and metabolic pathway studies.

PCK2 Antibody Human and Monkey Cell and Tissue WB. Western blot analysis was performed under reducing conditions using 30 ug of lysate per lane. Lane 1: human T-47D cells; Lane 2: human U-2 OS cells; Lane 3: human A431 cells; Lane 4: human K562 cells; Lane 5: monkey COS-7 cells; Lane 6: monkey lung tissue. The membrane was incubated overnight at 4°C with rabbit anti-PCK2 antibody at 0.5 ug/ml, followed by an HRP-conjugated secondary antibody and ECL detection. A specific band was observed at approximately 71 kDa. PCK2 Antibody, a mitochondrial gluconeogenesis enzyme antibody, detects endogenous PCK2 across human and monkey samples.

PCK2 Antibody Rat and Mouse Tissue WB. Western blot analysis was performed under reducing conditions using 30 ug of tissue lysate per lane. Lane 1: rat kidney; Lane 2: rat liver; Lane 3: mouse lung; Lane 4: mouse spleen; Lane 5: mouse kidney; Lane 6: mouse liver. The membrane was incubated overnight at 4°C with rabbit anti-PCK2 antibody at 0.5 ug/ml, followed by an HRP-conjugated secondary antibody and ECL detection. A specific band was observed at approximately 71 kDa in each sample, with variable expression intensity. PCK2 Antibody, a mitochondrial gluconeogenesis enzyme antibody, detects endogenous PCK2 across rat and mouse tissues.

PCK2 Antibody MCF7 Breast Cancer Cell FACS. Intracellular flow cytometry analysis was performed using human MCF7 breast cancer cells fixed with 4% paraformaldehyde and permeabilized before staining. Cells were blocked with 10% normal goat serum and incubated with rabbit anti-PCK2 antibody at 1 ug per million cells for 30 minutes at 20°C, followed by a Fluoro488-conjugated goat anti-rabbit IgG secondary antibody. The blue trace represents PCK2 staining, the green trace represents the isotype control and the red trace represents unstained cells. The rightward shift demonstrates detectable intracellular PCK2 expression. PCK2 Antibody, a mitochondrial gluconeogenesis enzyme antibody, supports metabolic analysis by flow cytometry.

Availability	1-3 business days
Species Reactivity	Human, Mouse, Rat, Monkey
Format	Antigen affinity purified
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Purity	Affinity purified
Buffer	Lyophilized from 1X PBS with 2% Trehalose
UniProt	Q16822
Applications	Western Blot : 0.5-1ug/ml Immunohistochemistry : 2-5ug/ml Immunofluorescence : 5ug/ml Flow Cytometry : 1-3ug/million cells Direct ELISA : 0.1-0.5ug/ml
Limitations	This PCK2 Antibody / Mitochondrial Gluconeogenesis Enzyme Antibody is available for research use only.
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Description

PCK2 Antibody recognizes phosphoenolpyruvate carboxykinase 2, a mitochondrial metabolic enzyme commonly known as PEPCK-M. PCK2 catalyzes the guanosine triphosphate-dependent conversion of oxaloacetate into phosphoenolpyruvate, carbon dioxide and guanosine diphosphate. This reaction connects mitochondrial tricarboxylic acid cycle activity with pathways that use phosphoenolpyruvate and other biosynthetic intermediates. PCK2 is encoded by the PCK2 gene on human chromosome 14 and is synthesized with a mitochondrial targeting sequence that directs the protein to the mitochondrial matrix. Its mitochondrial localization distinguishes PCK2 from PCK1, the cytosolic phosphoenolpyruvate carboxykinase isoform that is strongly associated with hepatic and renal gluconeogenesis.

PCK2 contributes to gluconeogenesis by supporting the movement of oxaloacetate-derived carbon from mitochondria toward cytosolic glucose production. However, its biological function extends beyond classical glucose synthesis. The enzyme participates in cataplerosis, the removal of metabolic intermediates from the tricarboxylic acid cycle for use in other cellular pathways. Through the generation of phosphoenolpyruvate, PCK2 can help direct carbon toward glyceroneogenesis, amino acid metabolism and the production of biosynthetic precursors. A PCK2 Antibody is therefore useful for investigating how mitochondrial carbon metabolism is coordinated with nutrient availability, energy demand and anabolic growth.

PCK2 expression and activity may become particularly important under conditions of limited glucose or other forms of metabolic stress. In nutrient-challenged cells, PCK2 can support metabolic flexibility by enabling selected tricarboxylic acid cycle intermediates to be redirected into pathways needed for cell survival and proliferation. Research has connected altered PCK2 regulation with cancer metabolism, endoplasmic reticulum stress responses, lipid synthesis, insulin secretion and metabolic adaptation in diverse tissues. Depending on the biological setting, changes in PCK2 abundance can influence glucose utilization, mitochondrial carbon flow and the balance between energy production and macromolecule synthesis. These context-dependent functions make PCK2 a relevant target in studies of tumor biology, diabetes, mitochondrial metabolism and cellular responses to nutrient stress.

PCK2 is expressed in multiple tissues, with prominent expression reported in the gastrointestinal tract and detectable expression in several metabolically active organs. Protein abundance and metabolic function can vary according to tissue type, developmental state and nutrient conditions. Researchers may use PCK2 Antibody products to examine endogenous PCK2 expression by western blot, immunohistochemistry, immunofluorescence or other validated antibody-based applications. Comparison of PCK2 with PCK1 can help distinguish mitochondrial and cytosolic phosphoenolpyruvate carboxykinase pathways, while analysis under fed, fasting, hypoxic or nutrient-limited conditions can reveal changes in metabolic adaptation. NSJ Bioreagents supplies PCK2 Antibody options for research into mitochondrial gluconeogenesis, cataplerosis, cancer metabolism and related pathways involving phosphoenolpyruvate production.

Explore our Metabolism Antibodies page for additional reagents targeting gluconeogenesis, mitochondrial carbon metabolism and cellular energy regulation.

Application Notes

Optimal dilution of the PCK2 Antibody / Mitochondrial Gluconeogenesis Enzyme Antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids M1-M640) was used as the immunogen for the PCK2 antibody.

Storage

After reconstitution, the PCK2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

PEPCK-M Antibody, PEPCK2 Antibody, Mitochondrial PEPCK Antibody, Phosphoenolpyruvate Carboxykinase 2 Antibody, Mitochondrial Phosphoenolpyruvate Carboxykinase Antibody, mtPCK2 Antibody

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