PAX8 Antibody for IF / Paired Box Protein Pax-8 Immunofluorescence Antibody (FY12105)

PAX8 Antibody for IF / Paired Box Protein Pax-8 Immunofluorescence Antibody immunofluorescence analysis in human HeLa cells showing strong nuclear localization of Paired box protein Pax-8 (PAX8). The rabbit polyclonal PAX8 antibody produces a distinct nuclear fluorescence signal (green), clearly delineating nuclei, while Beta Tubulin highlights the cytoplasmic microtubule network (red) as a structural reference. The merged image demonstrates sharp nuclear green staining surrounded by red cytoplasmic signal, enabling clear cell boundary definition and subcellular localization assessment. This pattern is consistent with the role of PAX8 as a nuclear transcription factor and supports its use in multiplex immunofluorescence applications for precise cellular and spatial analysis.

Western blot analysis of PAX8/Paired box gene 8 using anti-PAX8 antibody. Lane 1: human SW579 whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat NRK whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX8 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A specific band was detected for PAX8 at approximately 48 kDa. The expected band size for PAX8 is at 48 kDa.