PAX8 Antibody / Differentiated vs Dedifferentiated Tumor Marker Antibody (F49929)

PAX8 Antibody / Differentiated vs Dedifferentiated Tumor Marker Antibody western blot analysis in human HL-60 cell lysate. A band is detected at approximately 55-60 kDa, above the predicted molecular weight of Paired box protein Pax-8 (PAX8) at ~48 kDa. The upward shift in apparent molecular weight is consistent with post-translational modification or altered electrophoretic mobility of this nuclear transcription factor. This banding pattern supports detection of PAX8 and aligns with known variability in migration associated with transcriptional regulation and isoform expression.

PAX8 Antibody / Differentiated vs Dedifferentiated Tumor Marker Antibody immunohistochemistry in mouse kidney tissue showing predominantly cytoplasmic HRP-DAB brown staining with scattered nuclear positivity in renal tubular epithelial cells. Paired box protein Pax-8 (PAX8) is primarily a nuclear transcription factor, and the presence of cytoplasmic staining may reflect altered localization, isoform-specific distribution, or tissue-specific processing in this sample. Tubular epithelial structures are clearly highlighted, while surrounding stromal elements remain comparatively weak or negative. The mixed cytoplasmic and nuclear pattern provides context for interpreting differentiation status, as changes in subcellular localization can be associated with altered transcriptional activity or cellular state.

Flow cytometric analysis of 293 cells using PAX8 antibody (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.