PAX8 Antibody / Tumor Origin Identification Marker Antibody [clone PAX8/1491 + PAX8/1492] (V3414)

PAX8 Antibody / Tumor Origin Identification Marker Antibody immunohistochemistry in human renal cell carcinoma tissue showing strong nuclear HRP-DAB brown staining in tumor epithelial cells. Paired box protein Pax-8 (PAX8) expression is localized to nuclei of carcinoma cells forming glandular and tubular structures, with clear contrast against surrounding stromal elements that remain largely negative. The staining pattern supports renal epithelial origin, with dense nuclear positivity consistent across tumor cell populations, aiding tumor lineage identification in carcinoma specimens. Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 minutes.

PAX8 Antibody / Tumor Origin Identification Marker Antibody western blot analysis in human samples. Lane 1: human partial recombinant protein, Lane 2: human Raji cell lysate. A band is detected at approximately 48 kDa, consistent with the predicted molecular weight of Paired box protein Pax-8 (PAX8). Additional bands between ~31-43 kDa correspond to known PAX8 isoforms, while a higher band near ~62 kDa may reflect post-translationally modified forms of the protein or altered electrophoretic mobility. The observed banding pattern supports detection of multiple PAX8 isoforms and aligns with expected transcription factor expression in human cells.