PAX7 Antibody / Satellite Cell Marker Antibody [clone PAX7/1187] (V2773)

Description

PAX7 Antibody / Satellite Cell Marker Antibody recognizes PAX7, a paired box transcription factor with essential roles in skeletal muscle development, muscle progenitor cell specification and maintenance of the adult satellite cell population. PAX7 belongs to the PAX family of developmental transcription factors and contains a conserved paired DNA-binding domain and homeodomain that regulate target gene expression. In skeletal muscle, PAX7 is particularly associated with satellite cells, the resident stem and progenitor cells responsible for muscle growth, maintenance and regeneration. Its characteristic expression in this population has established PAX7 as one of the most widely studied markers of skeletal muscle satellite cells.

During embryonic development, PAX7 participates in the specification and maintenance of myogenic progenitor populations that contribute to formation of skeletal muscle. In adult muscle, quiescent satellite cells typically express PAX7 and reside between the basal lamina and sarcolemma of muscle fibers. Following muscle injury or other regenerative stimuli, satellite cells become activated, proliferate and generate myogenic progenitors capable of differentiating and contributing to new or repaired muscle fibers. A PAX7 Antibody is therefore useful for research examining satellite cell identity, muscle stem cell populations, myogenesis and skeletal muscle regeneration.

PAX7 expression is dynamically regulated as satellite cells progress through activation and differentiation. PAX7-positive progenitors can coexpress myogenic regulatory factors such as MYOD1 during early stages of activation, while subsequent differentiation is accompanied by changes in the expression of PAX7 and other transcriptional regulators. These coordinated expression patterns are widely studied to characterize different stages of the myogenic lineage. As a Satellite Cell Marker Antibody target, PAX7 is particularly relevant to investigations of satellite cell abundance, self-renewal, lineage progression and responses to muscle injury, aging or experimental manipulation.

PAX7 is also relevant to cancer research, particularly rhabdomyosarcoma, a malignant tumor displaying features of skeletal muscle differentiation. Alterations involving PAX family transcription factors occur in subsets of rhabdomyosarcoma, and PAX7 expression can provide information about myogenic lineage and tumor biology. PAX7 has consequently been investigated alongside other skeletal muscle-associated transcription factors and differentiation markers in studies of rhabdomyosarcoma and related tumors. Its expression in additional neoplastic tissues may also provide opportunities to investigate how developmental transcription programs are retained or reactivated during tumorigenesis.

The biological importance of PAX7 extends beyond its use as a cellular marker. Experimental studies of PAX7 have helped define mechanisms controlling satellite cell specification, maintenance, proliferation and differentiation, as well as the transcriptional programs underlying skeletal muscle development and regeneration. The protein is therefore relevant to research spanning developmental biology, stem cell biology, regenerative medicine, neuromuscular research and cancer biology. NSJ Bioreagents supplies antibodies to PAX7 and other proteins associated with skeletal muscle development and myogenic differentiation. The mouse monoclonal PAX7/1187 clone provides a defined antibody reagent for investigating PAX7 expression in experimental samples.

For related targets involved in muscle stem cell maintenance, differentiation and tissue regeneration, explore our Stem Cell Antibodies page.

Application Notes

Optimal dilution of the PAX7 Antibody / Satellite Cell Marker Antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human PAX7 protein corresponding to amino acids 301-505 was used as the immunogen for this antibody.

Storage

Store the PAX7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

PAX7 antibody, Paired box protein 7 antibody, Paired box 7 antibody, Satellite cell marker antibody, Muscle satellite cell marker antibody, Muscle stem cell marker antibody