PAX7 Antibody / Paired Box Protein 7 Antibody (FY12505)

PAX7 Antibody Caco-2 Cell FACS. Flow cytometry analysis of fixed and permeabilized human Caco-2 cells using PAX7 Antibody. Cells were blocked with 10% normal goat serum and incubated with the antibody at 1 ug/million cells for 30 minutes at 20°C, followed by DyLight 488-conjugated goat anti-rabbit IgG. The histogram shows PAX7 staining (blue), isotype control (green) and unstained cells (red). A clear fluorescence shift is observed with PAX7 staining relative to the isotype control. This PAX7 Antibody, also called Paired Box Protein 7 Antibody, detects intracellular PAX7 in human cells by flow cytometry.

PAX7 Antibody Mouse and Rat Brain WB. Western blot analysis of PAX7 in rat and mouse brain tissue lysates using PAX7 Antibody. Samples were separated by 10% SDS-PAGE and transferred to nitrocellulose. Lane 1: rat brain; Lane 2: mouse brain. The membrane was blocked with 5% non-fat milk/TBS and incubated with the antibody at 0.5 ug/ml overnight at 4°C, followed by HRP-conjugated goat anti-rabbit IgG and ECL detection. PAX7 was detected as a major band at approximately 55 kDa, consistent with its predicted molecular weight, and a slower-migrating band at approximately 65 kDa, consistent with phosphorylation-dependent modification of PAX7 reported in previous studies. This PAX7 Antibody, also called Paired Box Protein 7 Antibody, detects PAX7 in mouse and rat brain tissue.