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Home >> Antibodies >> PAX6 Antibody

PAX6 Antibody (R32035)

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  Catalog No Formulation Size Price (USD)  
Image R32035 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat brain, 2) mouse brain, 3) human U87 and 4) human HeLa lysate with PAX6 antibody. Expected/observed molecular weight ~48 kDa.
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Western blot testing of 1) rat brain, 2) mouse brain, 3) human U87 and 4) human HeLa lysate with PAX6 antibody. Expected/observed molecular weight ~48 kDa.
IHC testing of FFPE human pancreatic cancer tissue with PAX6 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE mouse pancreas with PAX6 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat pancreas with PAX6 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P26367
Localization Nuclear, cytoplasmic
Applications Western blot : 0.1-0.5ug/ml
IHC (FFPE) : 0.5-1ug/ml
Limitations This PAX6 antibody is available for research use only.
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Description

Paired box protein Pax-6, also known as aniridia type II protein (AN2) or oculorhombin, is a protein that in humans is encoded by the PAX6 gene. This gene encodes a homeobox and paired domain-containing protein that binds DNA and functions as a regulator of transcription. Activity of this protein is key in the development of neural tissues, particularly the eye. In addition, this gene is regulated by multiple enhancers located up to hundreds of kilobases distant from this locus. Mutations in this gene or in the enhancer regions can cause ocular disorders such as aniridia and Peter's anomaly. Use of alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms.

Application Notes

Optimal dilution of the PAX6 antibody should be determined by the researcher.

Immunogen

Amino acids EDSDEAQMRLQLKRKLQRNRTSFTQEQIEALEKEFERTH of human PAX6 were used as the immunogen for the PAX6 antibody.

Storage

After reconstitution, the PAX6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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