- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
PAX6 Antibody / Knockdown-Validated Transcription Factor Antibody [clone PAX6/8578R] (V4145)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V4145-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
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V4145-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
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V4145SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Clone Name | PAX6/8578R |
| Purity | Protein A/G affinity |
| UniProt | P26367 |
| Localization | Nucleus |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT Western Blot : 2-4ug/ml |
| Limitations | This PAX6 Antibody / Knockdown-Validated Transcription Factor Antibody is available for research use only. |
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Paired box protein Pax-6 (PAX6) is a nuclear transcription factor belonging to the paired box (PAX) family, characterized by the presence of both a paired DNA-binding domain and a homeobox domain that enable precise regulation of gene expression. PAX6 (PAX6) is a master regulator of developmental programs, with well-established roles in ocular formation, central nervous system patterning, and pancreatic endocrine differentiation. In adult tissues, its expression becomes more restricted but remains biologically significant in specialized cell populations, particularly within endocrine and neuroectoderm-derived lineages. The PAX6 Antibody / Knockdown-Validated Transcription Factor Antibody is designed to detect this critical regulatory protein with high specificity, supported by both functional validation and consistent expression profiling. This antibody is part of a collection of knockdown validated antibodies that have been functionally assessed using gene silencing approaches to support target-specific detection.
PAX6 antibody, also referred to as Paired box protein Pax-6 antibody and PAX6 transcription factor antibody, recognizes a protein that localizes predominantly to the nucleus, consistent with its function as a transcriptional regulator. Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human pancreas tissue demonstrates strong and selective nuclear HRP-DAB brown staining in endocrine cells within pancreatic islets. Surrounding exocrine tissue shows minimal to no staining, providing a clear contrast that reflects the lineage-restricted expression of PAX6. This highly specific nuclear pattern allows for confident identification of islet cell populations and supports use in studies of endocrine differentiation and tissue organization.
Western blot analysis identifies a distinct band at approximately 45-50 kDa in human cell lysates, consistent with the expected molecular weight of PAX6. Importantly, knockdown validation using PAX6-targeted shRNA in HeLa cells results in a clear and reproducible reduction of signal compared to wild-type controls. This gene silencing-based approach provides direct functional confirmation that the detected band corresponds to PAX6 protein, establishing a strong link between antibody signal and target expression. The inclusion of knockdown validation places this clone among a smaller subset of antibodies with experimentally demonstrated specificity at the protein level.
Functionally, PAX6 regulates transcriptional networks that control cell identity, proliferation, and differentiation. In the pancreas, PAX6 is essential for the development and maintenance of hormone-producing islet cells, including insulin-secreting beta cells and other endocrine subtypes. Its nuclear expression in these cells reflects active transcriptional control of endocrine-specific genes and is a defining feature observed in IHC. In neural tissues, PAX6 contributes to progenitor cell regulation and regional patterning, further underscoring its role as a lineage-determining factor.
The combination of highly specific nuclear IHC staining, strong western blot performance, and functional knockdown validation makes clone PAX6/8578R a well-characterized reagent for studying transcription factor biology. The clear reduction of signal in knockdown conditions, together with distinct nuclear localization in tissue sections, supports its use in applications requiring high confidence in target specificity, including studies of developmental pathways, endocrine biology, and gene regulation.
This antibody is part of a broader antibody panel offered by NSJ Bioreagents.
Optimal dilution of the PAX6 Antibody / Knockdown-Validated Transcription Factor Antibody should be determined by the researcher.
A recombinant partial protein (within amino acids 1-300) from the human protein was used as the immunogen for the PAX6 antibody.
Aliquot the PAX6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
PAX6 antibody, Pax-6 antibody, paired box protein Pax-6 antibody, aniridia type II protein antibody
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