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Home >> Antibodies >> PAX3 Antibody

PAX3 Antibody (RQ5907)

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  Catalog No Formulation Size Price (USD)  
Image RQ5907 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human U-87 MG cells with PAX3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PAX3 antibody.
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Flow cytometry testing of human U-87 MG cells with PAX3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PAX3 antibody.
Western blot testing of 1) human SH-SY5Y, 2) human K562, 3) human SW620, 4) rat brain and 5) mouse NIH 3T3 lysate with PAX3 antibody. Expected molecular weight: 22-56 kDa (multiple isoforms).
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P23760
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This PAX3 antibody is available for research use only.
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Description

This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini.

Application Notes

Optimal dilution of the PAX3 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids T301-F479) was used as the immunogen for the PAX3 antibody.

Storage

After reconstitution, the PAX3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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