PAX2 Antibody PAX2/1104 | Mouse Monoclonal

	Catalog No	Formulation	Size	Price (USD)
	V7450-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	559
Formulation
	V7450-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	259
Formulation
	V7450SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	559
Formulation

PAX2 Antibody PAX2/1104 Protein Microarray Validation. Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using PAX2 Antibody clone PAX2/1104 demonstrates strong preferential recognition of PAX2. PAX2 ranked first with a Z-score of 86.89 and an S-score of 74.72. The Z-score represents signal strength in standard deviations above the array mean, while the S-score measures separation from the next highest-ranked protein and indicates relative target specificity. These results demonstrate the high specificity of PAX2 Antibody clone PAX2/1104 for PAX2 in human protein microarray testing.

PAX2 Antibody PAX2/1104 Human Colon Cancer IHC. Immunohistochemical staining of FFPE human colon carcinoma tissue with PAX2 Antibody clone PAX2/1104 demonstrates prominent nuclear PAX2 expression. Heat-induced epitope retrieval was performed by boiling sections in pH9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling. Strong brown nuclear staining is observed in tumor cells, consistent with the nuclear localization of the PAX2 transcription factor. This PAX2 Antibody mouse monoclonal demonstrates the staining characteristics of clone PAX2/1104 in human colon carcinoma tissue.

PAX2 Antibody PAX2/1104 Human Ovarian Cancer IHC. Immunohistochemical staining of FFPE human ovarian carcinoma tissue with PAX2 Antibody clone PAX2/1104 demonstrates prominent nuclear PAX2 expression. Heat-induced epitope retrieval was performed by boiling sections in pH9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling. Strong brown nuclear staining is observed in tumor cells, consistent with the nuclear localization of the PAX2 transcription factor. This PAX2 Antibody mouse monoclonal demonstrates the staining characteristics of clone PAX2/1104 in human ovarian carcinoma tissue.

PAX2 Antibody PAX2/1104 Human Renal Cell Carcinoma IHC. Immunohistochemical staining of FFPE human renal cell carcinoma tissue with PAX2 Antibody clone PAX2/1104 demonstrates nuclear PAX2 expression. Heat-induced epitope retrieval was performed by boiling sections in pH9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling. Brown nuclear staining is observed in tumor cells, consistent with the nuclear localization of the PAX2 transcription factor. This PAX2 Antibody mouse monoclonal demonstrates the staining characteristics of clone PAX2/1104 in human renal cell carcinoma tissue.

SDS-PAGE analysis of purified, BSA-free PAX2 antibody (clone PAX2/1104) as confirmation of integrity and purity.

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PAX2 Antibody PAX2/1104 Protein Microarray Validation. Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using PAX2 Antibody clone PAX2/1104 demonstrates strong preferential recognition of PAX2. PAX2 ranked first with a Z-score of 86.89 and an S-score of 74.72. The Z-score represents signal strength in standard deviations above the array mean, while the S-score measures separation from the next highest-ranked protein and indicates relative target specificity. These results demonstrate the high specificity of PAX2 Antibody clone PAX2/1104 for PAX2 in human protein microarray testing.

PAX2 Antibody PAX2/1104 Human Colon Cancer IHC. Immunohistochemical staining of FFPE human colon carcinoma tissue with PAX2 Antibody clone PAX2/1104 demonstrates prominent nuclear PAX2 expression. Heat-induced epitope retrieval was performed by boiling sections in pH9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling. Strong brown nuclear staining is observed in tumor cells, consistent with the nuclear localization of the PAX2 transcription factor. This PAX2 Antibody mouse monoclonal demonstrates the staining characteristics of clone PAX2/1104 in human colon carcinoma tissue.

PAX2 Antibody PAX2/1104 Human Ovarian Cancer IHC. Immunohistochemical staining of FFPE human ovarian carcinoma tissue with PAX2 Antibody clone PAX2/1104 demonstrates prominent nuclear PAX2 expression. Heat-induced epitope retrieval was performed by boiling sections in pH9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling. Strong brown nuclear staining is observed in tumor cells, consistent with the nuclear localization of the PAX2 transcription factor. This PAX2 Antibody mouse monoclonal demonstrates the staining characteristics of clone PAX2/1104 in human ovarian carcinoma tissue.

PAX2 Antibody PAX2/1104 Human Renal Cell Carcinoma IHC. Immunohistochemical staining of FFPE human renal cell carcinoma tissue with PAX2 Antibody clone PAX2/1104 demonstrates nuclear PAX2 expression. Heat-induced epitope retrieval was performed by boiling sections in pH9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling. Brown nuclear staining is observed in tumor cells, consistent with the nuclear localization of the PAX2 transcription factor. This PAX2 Antibody mouse monoclonal demonstrates the staining characteristics of clone PAX2/1104 in human renal cell carcinoma tissue.

SDS-PAGE analysis of purified, BSA-free PAX2 antibody (clone PAX2/1104) as confirmation of integrity and purity.

Availability	1-3 business days
Species Reactivity	Human
Format	Purified
Host	Mouse
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG2b, kappa
Clone Name	PAX2/1104
Purity	Protein G affinity chromatography
UniProt	Q02962
Localization	Nuclear
Applications	ELISA : order BSA/sodium azide-free format for coating Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations	This PAX2 Antibody PAX2/1104 is available for research use only.
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Description

PAX2 Antibody PAX2/1104 is a mouse monoclonal that recognizes PAX2, a nuclear transcription factor belonging to the paired box family of developmental regulatory proteins. PAX2 contains a conserved paired DNA-binding domain and regulates genes involved in embryonic development, cellular differentiation, and organogenesis. Its expression is particularly important during development of the kidney and urogenital tract, with additional roles in development of selected structures of the nervous system, eye, and ear. The tightly regulated expression of PAX2 reflects its importance in establishing and maintaining specific developmental cell lineages.

PAX2 plays a central role in renal development, including specification of the nephric lineage and formation of structures that contribute to the developing kidney. Expression is prominent in embryonic renal tissues and becomes substantially restricted in most mature kidney structures. Alterations affecting PAX2 expression or function can interfere with normal renal and ocular development. A PAX2 Antibody can therefore support investigations of kidney organogenesis, renal cell differentiation, developmental transcription programs, and urogenital biology.

PAX2 expression is also studied extensively in cancer biology. Persistent or reactivated PAX2 expression occurs in several tumor types, particularly malignancies associated with renal and Müllerian lineages. Its lineage-associated expression has made PAX2 relevant to research involving renal cell carcinoma and selected ovarian and endometrial tumors. PAX2 may also participate in transcriptional programs affecting tumor cell survival, proliferation, and differentiation. Clone PAX2/1104 provides a mouse monoclonal reagent for investigating PAX2 expression in developmental and cancer research.

Clone PAX2/1104 has been evaluated by human protein microarray analysis to assess recognition of PAX2 relative to a broad panel of human proteins. This proteome-wide screening provides an additional measure of target specificity that complements application-specific validation. The clone also has documented use in published research. NSJ Bioreagents offers PAX2/1104 for research into developmental transcription, renal biology, and cancer. This PAX2 Antibody clone provides a Protein Microarray Validated mouse monoclonal reagent for investigating PAX2 expression and Paired Box 2-associated biology.

For additional information on PAX2 biology and available antibodies, see our PAX2 Antibody / Paired Box 2 Antibody page.

Application Notes

Optimal dilution of the PAX2 Antibody PAX2/1104 should be determined by the researcher.

Immunogen

A portion of amino acids 223-354 from the human protein was used as the immunogen for this PAX2 antibody.

Storage

Store the PAX2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

PAX2 antibody, Paired Box 2 antibody, PAX-2 antibody, Paired Box Gene 2 antibody, PAX2/1104 antibody

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PAX2 Antibody PAX2/1104 / Mouse Monoclonal [clone PAX2/1104] (V7450)

Description

Application Notes

Immunogen

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NSJ Bioreagents