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Home >> Antibodies >> PAX2 Antibody

PAX2 Antibody [clone PAX2/8671R] (V4114)

  Catalog No Formulation Size Price (USD)  
Image V4114-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4114-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4114SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human renal cell carcinoma tissue with PAX2 antibody (clone PAX2/8671R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name PAX2/8671R
Purity Protein A/G affinity
UniProt Q02962
Localization Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This PAX2 antibody is available for research use only.
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Description

Recognizes a protein of 42kDa, which is identified as PAX2. It is a member of the paired box family of transcription factors, which is required for development and proliferation of the kidney, brain, and mullerian organs. PAX2 genes contain a highly conserved DNA sequence within the paired box region, which encodes a DNA-binding domain, enabling PAX proteins to bind the promoters of specific genes to transcriptionally regulate their expression. PAX2 is specifically expressed in the developing central nervous system, eye, ear, and urogenital tract, and is essential for the development of these organs. In normal adult tissues PAX2 was mainly detected in the urogenital system, including kidney, ureteric epithelium, fallopian tube epithelium, ovary and uterus. In tumors, PAX2 has been detected in renal cell carcinomas, Wilms' tumors, nephrogenic adenomas and papillary serous carcinoma of the ovary. PAX2 has been used as a marker for the identification of renal cell carcinoma and ovarian carcinoma by immunohistochemistry.

Application Notes

Optimal dilution of the PAX2 antibody should be determined by the researcher.

Immunogen

A recombinant partial protein (within amino acids 223-354) from the human protein was used as the immunogen for the PAX2 antibody.

Storage

Aliquot the PAX2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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