PARP1 Antibody / Chromatin Repair Protein Antibody (R31722)

PARP1 Antibody HeLa KO Validation WB. Western blot analysis of human HeLa-WT and HeLa-GPX4 KO cell lysates using PARP1 antibody. A strong band is detected near the expected molecular weight of full-length PARP1 in both wild-type and GPX4 knockout samples, consistent with preserved expression of this chromatin-associated repair protein independent of GPX4 status. ACTB is shown as a loading control. The observed signal pattern supports robust detection of PARP1-associated DNA strand break signaling, nuclear stress-response pathways, and genomic stability maintenance in human cervical carcinoma cells.

PARP1 Antibody Human Placenta IHC. Immunohistochemistry analysis of FFPE human placenta tissue using PARP1 antibody. Placental trophoblast cell nuclei demonstrate HRP-DAB brown staining consistent with the chromatin-associated activity of PARP1 in DNA repair, genomic stability maintenance, and nuclear stress-response signaling pathways within actively remodeling placental tissue. Nuclei are counterstained blue. Heat-induced epitope retrieval was performed by steaming tissue sections in pH6 citrate buffer prior to staining.

PARP1 Antibody A549 ICC. Immunocytochemistry analysis of human A549 cells using PARP1 antibody. Cell nuclei demonstrate strong HRP-DAB brown staining consistent with the chromatin-associated localization of PARP1 in DNA strand break repair, genomic stability maintenance, and nuclear stress-response signaling pathways. Nuclei are counterstained blue.

PARP1 Antibody Multi-Species WB. Western blot analysis of human, rat, and mouse cell lysates using PARP1 antibody at 0.5 ug/ml. Lane 1: human HeLa lysate, Lane 2: human HepG2 lysate, Lane 3: human COLO-320 lysate, Lane 4: human Jurkat lysate, Lane 5: rat PC-12 lysate, Lane 6: mouse NIH 3T3 lysate, Lane 7: mouse HEPA 1-6 lysate. A predominant band is detected near 116 kDa, consistent with the predicted molecular weight of full-length PARP1. Additional lower molecular weight signal near approximately 55-89 kDa in selected samples may reflect proteolytic processing or cleavage-associated PARP1 fragments containing the catalytic domain. The observed expression pattern supports the widespread nuclear distribution of this chromatin-associated repair protein involved in DNA strand break signaling, genomic stability maintenance, and cellular stress-response pathways across multiple mammalian cell types.

PARP1 Antibody Rat Brain IHC. Immunohistochemistry analysis of FFPE rat brain tissue using PARP1 antibody. Neuronal and glial cell nuclei demonstrate HRP-DAB brown staining consistent with the chromatin-associated localization of PARP1 in DNA repair, genomic maintenance, and oxidative stress-response signaling pathways within neural tissue. Nuclei are counterstained blue. Heat-induced epitope retrieval was performed by steaming tissue sections in pH6 citrate buffer prior to staining.

PARP1 Antibody Mouse Intestine IHC. Immunohistochemistry analysis of FFPE mouse intestine tissue using PARP1 antibody. Intestinal epithelial cell nuclei demonstrate strong HRP-DAB brown staining consistent with the chromatin-associated role of PARP1 in DNA strand break repair, genomic maintenance, and nuclear stress-response signaling within rapidly proliferating epithelial tissue. Nuclei are counterstained blue. Heat-induced epitope retrieval was performed by steaming tissue sections in pH6 citrate buffer prior to staining.

PARP1 Antibody Bladder Cancer IF. Immunofluorescence analysis of FFPE human bladder cancer tissue using PARP1 antibody. PARP1 staining (red) demonstrates strong nuclear localization within tumor cell populations, consistent with the chromatin-associated role of this repair protein in genomic stability maintenance and nuclear stress-response signaling pathways in proliferative carcinoma tissue. Nuclei are counterstained blue with DAPI. Heat-induced epitope retrieval was performed by steaming tissue sections in pH8 EDTA buffer for 20 minutes prior to staining.

PARP1 Antibody Stomach Cancer IF. Immunofluorescence analysis of FFPE human stomach cancer tissue using PARP1 antibody. PARP1 staining (red) demonstrates predominantly nuclear localization within gastric tumor epithelial cells, consistent with the chromatin-associated activity of this repair protein in genomic surveillance and cellular stress-response signaling pathways. Nuclei are counterstained blue with DAPI. Heat-induced epitope retrieval was performed by steaming tissue sections in pH8 EDTA buffer for 20 minutes prior to staining.

PARP1 Antibody Pancreas Cancer IF. Immunofluorescence analysis of FFPE human pancreas cancer tissue using PARP1 antibody. PARP1 staining (red) demonstrates strong nuclear localization within pancreatic tumor epithelial cells, consistent with the chromatin-associated role of this repair protein in genomic maintenance and nuclear stress-response signaling pathways active in proliferative carcinoma tissue. Nuclei are counterstained blue with DAPI. Heat-induced epitope retrieval was performed by steaming tissue sections in pH8 EDTA buffer for 20 minutes prior to staining.

PARP1 Antibody Breast Cancer IF. Immunofluorescence analysis of FFPE human breast cancer tissue using PARP1 antibody. PARP1 staining (red) demonstrates widespread nuclear localization within tumor cell populations, consistent with the chromatin-associated activity of this repair protein in DNA strand break signaling and genomic stability maintenance pathways. Nuclei are counterstained blue with DAPI. Heat-induced epitope retrieval was performed by steaming tissue sections in pH8 EDTA buffer for 20 minutes prior to staining.

PARP1 Antibody Bladder Tissue IHC. Immunohistochemistry analysis of FFPE human bladder tissue using PARP1 antibody. Urothelial and stromal cell nuclei demonstrate HRP-DAB brown staining consistent with the chromatin-associated localization of PARP1 in DNA repair, genomic surveillance, and nuclear stress-response signaling pathways. Nuclei are counterstained blue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH8 EDTA buffer for 20 minutes followed by cooling prior to staining.

PARP1 Antibody Human Tonsil IHC. Immunohistochemistry analysis of FFPE human tonsil tissue using PARP1 antibody. Lymphoid cell nuclei demonstrate widespread HRP-DAB brown staining consistent with the chromatin-associated localization of PARP1 in DNA repair, genomic surveillance, and nuclear stress-response signaling pathways within proliferative immune cell populations. Nuclei are counterstained blue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH8 EDTA buffer for 20 minutes followed by cooling prior to staining.

PARP1 Antibody Mouse Liver IHC. Immunohistochemistry analysis of FFPE mouse liver tissue using PARP1 antibody. Hepatocyte nuclei demonstrate diffuse HRP-DAB brown staining consistent with the chromatin-associated role of PARP1 in DNA repair, oxidative stress adaptation, and genomic maintenance pathways within metabolically active hepatic tissue. Nuclei are counterstained blue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH8 EDTA buffer for 20 minutes followed by cooling prior to staining.

PARP1 Antibody Mouse Kidney IHC. Immunohistochemistry analysis of FFPE mouse kidney tissue using PARP1 antibody. Renal tubular epithelial cell nuclei demonstrate prominent HRP-DAB brown staining consistent with the chromatin-associated localization of PARP1 in DNA damage repair, genomic stability maintenance, and nuclear stress-response signaling pathways. Nuclei are counterstained blue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH8 EDTA buffer for 20 minutes followed by cooling prior to staining.

PARP1 Antibody Rat Kidney IHC. Immunohistochemistry analysis of FFPE rat kidney tissue using PARP1 antibody. Renal tubular epithelial cell nuclei demonstrate HRP-DAB brown staining consistent with the chromatin-associated activity of PARP1 in DNA strand break repair, genomic surveillance, and cellular stress-response signaling pathways within kidney tissue. Nuclei are counterstained blue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH8 EDTA buffer for 20 minutes followed by cooling prior to staining.