PARP1 Antibody DNA Repair Marker CFD-16

	Catalog No	Formulation	Size	Price (USD)
	RQ5290	Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA	100 ul	449
Formulation

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PARP1 Antibody Knockout Validation WB. Western blot analysis of human HeLa-WT and HeLa KO whole cell lysates using PARP1 antibody clone CFD-16. A strong band is detected near 118 kDa in wild-type HeLa lysate, consistent with the predicted molecular weight of full-length PARP1, while knockout lysate demonstrates complete loss of signal, supporting highly specific detection of this chromatin-associated DNA repair enzyme. ACTB is shown as a loading control. The observed knockout validation pattern supports the role of PARP1 in DNA strand break repair, genomic stability maintenance, and nuclear stress-response signaling pathways.

PARP1 Antibody Multi-Cell Line WB. Western blot analysis of human HeLa, HepG2, SIHA, K562, 293T, and SH-SY5Y whole cell lysates using recombinant rabbit monoclonal PARP1 antibody clone CFD-16. Lane 1: human HeLa lysate, Lane 2: human HepG2 lysate, Lane 3: human SIHA lysate, Lane 4: human K562 lysate, Lane 5: human 293T lysate, Lane 6: human SH-SY5Y lysate. A strong band is detected near 113 kDa across all tested samples, consistent with the predicted molecular weight of PARP1. The broad detection pattern supports the widespread expression of this chromatin-associated DNA repair enzyme involved in genomic stability maintenance, DNA strand break signaling, and nuclear stress response pathways across diverse human cell types.

PARP1 Antibody Mouse Tissue WB. Western blot analysis of mouse heart, liver, lung, kidney, brain, and skin tissue lysates using recombinant rabbit monoclonal PARP1 antibody clone CFD-16 at 1:1000 dilution for 1 hour at room temperature. Lane 1: mouse heart lysate, Lane 2: mouse liver lysate, Lane 3: mouse lung lysate, Lane 4: mouse kidney lysate, Lane 5: mouse brain lysate, Lane 6: mouse skin lysate. A prominent band is detected near 113 kDa across all tested tissues, consistent with the predicted molecular weight of PARP1. The broad tissue distribution supports the role of this chromatin-associated DNA repair enzyme in genomic stability maintenance, nuclear stress surveillance, and DNA strand break signaling across metabolically active and proliferative mouse tissues.

PARP1 Antibody HeLa IF. Immunofluorescence analysis of human HeLa cells using recombinant rabbit monoclonal PARP1 antibody clone CFD-16. PARP1 staining (green) demonstrates strong nuclear localization consistent with the chromatin-associated distribution of this DNA repair enzyme involved in genomic stability maintenance and DNA strand break response signaling. Nuclei are counterstained blue with DAPI.

PARP1 Antibody Stomach Cancer IHC. Immunohistochemistry analysis of FFPE human stomach cancer tissue using recombinant rabbit monoclonal PARP1 antibody clone CFD-16. Tumor epithelial cell nuclei demonstrate strong HRP-DAB brown staining consistent with the chromatin-associated localization of PARP1 in DNA strand break repair, genomic surveillance, and nuclear stress-response signaling pathways within proliferative gastric carcinoma tissue. Nuclei are counterstained blue. Heat-mediated antigen retrieval was performed in pH8 EDTA buffer prior to staining.

PARP1 Antibody Human Tonsil IHC. Immunohistochemistry analysis of FFPE human tonsil tissue using recombinant rabbit monoclonal PARP1 antibody clone CFD-16. Lymphoid cells demonstrate widespread nuclear HRP-DAB brown staining consistent with the role of PARP1 as a chromatin-associated DNA repair enzyme involved in genomic surveillance, stress-response signaling, and proliferative nuclear activity within immune cell populations. Nuclei are counterstained blue. Heat-mediated antigen retrieval was performed in pH8 EDTA buffer prior to staining.

PARP1 Antibody Bladder Cancer IHC. Immunohistochemistry analysis of FFPE human bladder cancer tissue using recombinant rabbit monoclonal PARP1 antibody clone CFD-16. Tumor cell nuclei demonstrate HRP-DAB brown staining consistent with the chromatin-associated localization of PARP1 in DNA damage repair, genomic stability regulation, and nuclear stress-response signaling pathways commonly active in proliferative tumor tissue. Nuclei are counterstained blue. Heat-mediated antigen retrieval was performed in pH8 EDTA buffer prior to staining.

PARP1 Antibody Pancreas Cancer IHC. Immunohistochemistry analysis of FFPE human pancreas cancer tissue using recombinant rabbit monoclonal PARP1 antibody clone CFD-16. Tumor cell nuclei demonstrate HRP-DAB brown staining consistent with the nuclear localization of PARP1 in chromatin-associated DNA repair enzyme pathways involved in genomic stability maintenance and cellular stress-response signaling within pancreatic carcinoma tissue. Nuclei are counterstained blue. Heat-mediated antigen retrieval was performed in pH8 EDTA buffer prior to staining.

PARP1 Antibody Mouse Kidney IHC. Immunohistochemistry analysis of FFPE mouse kidney tissue using recombinant rabbit monoclonal PARP1 antibody clone CFD-16. Renal tubular epithelial cells and scattered interstitial cell populations demonstrate prominent nuclear HRP-DAB brown staining consistent with the chromatin-associated localization of PARP1 in DNA repair, genomic stability maintenance, and nuclear stress response signaling pathways. Nuclei are counterstained blue. Heat-mediated antigen retrieval was performed in pH8 EDTA buffer prior to staining.

PARP1 Antibody Rat Kidney IHC. Immunohistochemistry analysis of FFPE rat kidney tissue using recombinant rabbit monoclonal PARP1 antibody clone CFD-16. Renal tubular epithelial cells demonstrate prominent nuclear HRP-DAB brown staining consistent with the chromatin-associated localization of PARP1 in DNA strand break repair and genomic stability maintenance pathways, while surrounding stromal regions show comparatively lower signal intensity. Nuclei are counterstained blue. Heat-mediated antigen retrieval was performed in pH8 EDTA buffer prior to staining.

PARP1 Antibody Rat Liver IHC. Immunohistochemistry analysis of FFPE rat liver tissue using PARP1 Antibody / Chromatin Repair Protein Antibody. Hepatocyte nuclei demonstrate diffuse HRP-DAB brown staining consistent with the chromatin-associated localization of PARP1 in DNA repair, genomic stability maintenance, and oxidative stress-response signaling pathways within metabolically active hepatic tissue. Nuclei are counterstained blue. Heat-induced epitope retrieval was performed using EDTA buffer, pH8.0, prior to staining.

PARP1 Antibody Pancreas Cancer IF. Immunofluorescence analysis of FFPE human pancreas cancer tissue using recombinant rabbit monoclonal PARP1 antibody clone CFD-16. PARP1 staining (red) demonstrates strong nuclear localization within tumor epithelial cells, consistent with the chromatin-associated role of this DNA repair enzyme in genomic stability maintenance and nuclear stress-response signaling pathways in pancreatic carcinoma tissue. Nuclei are counterstained blue with DAPI. Heat-mediated antigen retrieval was performed in pH8 EDTA buffer prior to staining.

PARP1 Antibody Stomach Cancer IF. Immunofluorescence analysis of FFPE human stomach cancer tissue using recombinant rabbit monoclonal PARP1 antibody clone CFD-16. PARP1 staining (red) demonstrates predominantly nuclear localization within gastric tumor epithelial cells, consistent with the role of this chromatin-associated DNA repair enzyme in genomic surveillance, DNA strand break signaling, and nuclear stress-response pathways in proliferative carcinoma tissue. Nuclei are counterstained blue with DAPI. Heat-mediated antigen retrieval was performed in pH8 EDTA buffer prior to staining.

Availability	1-2 weeks
Species Reactivity	Human
Format	Purified
Host	Rabbit
Clonality	Rabbit Monoclonal
Isotype	Rabbit IgG
Clone Name	CFD-16
Purity	Affinity purified
UniProt	P09874
Applications	Western Blot : 1:500-1:2000 Immunohistochemistry (FFPE) : 1:50-1:200 Immunofluorescence : 1:50-1:200
Limitations	This PARP1 Antibody / DNA Repair Enzyme Antibody is available for research use only.
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Description

Poly [ADP-ribose] polymerase 1 (PARP1) is a nuclear enzyme encoded by the PARP1 gene that functions as a central regulator of DNA damage detection, chromatin remodeling, genomic stability, and stress-responsive signaling pathways. PARP1 Antibody / DNA Repair Enzyme Antibody is useful for investigating DNA strand break repair, transcriptional regulation, apoptosis, and chromatin-associated signaling mechanisms. PARP1 antibody, also referred to as PARP antibody, Poly ADP-ribose polymerase 1 antibody, ADPRT antibody, or Nuclear DNA repair protein antibody in the literature, recognizes one of the most extensively studied DNA repair enzymes involved in cellular stress adaptation and genome maintenance. Recombinant rabbit monoclonal clone CFD-16 supports investigation of PARP1 expression in oncology, neurobiology, inflammation, and DNA repair-associated research applications.

PARP1 rapidly binds DNA strand breaks through zinc finger DNA-binding domains and catalyzes poly-ADP ribosylation of target proteins involved in chromatin relaxation and DNA repair complex recruitment. PARylation signaling mediated by PARP1 regulates base excision repair, replication stress adaptation, transcriptional control, cell cycle progression, and programmed cell death pathways. PARP1 additionally participates in inflammatory signaling, oxidative stress responses, telomere maintenance, and epigenetic regulation through interactions with histones, transcription factors, and chromatin remodeling proteins. Because PARP1 integrates DNA repair with broader stress-response signaling networks, it is considered a major nuclear regulatory enzyme across multiple biologic systems.

Aberrant PARP1 activation has been associated with cancer progression, genomic instability, neurodegeneration, ischemic injury, chronic inflammation, and metabolic stress signaling. Tumor cells frequently depend on PARP1-mediated DNA repair adaptation to survive replication-associated genomic damage, making PARP1 an important therapeutic target in oncology. PARP inhibitors are now widely used in cancers characterized by homologous recombination repair defects, particularly BRCA-mutated ovarian, breast, prostate, and pancreatic malignancies. PARP1 signaling is also highly relevant in studies of chemotherapy resistance, radiation response, and synthetic lethality-associated therapeutic strategies.

At the cellular level, PARP1 is predominantly localized within the nucleus where it associates with chromatin and DNA repair complexes. Immunohistochemistry and immunofluorescence studies typically demonstrate strong nuclear staining patterns consistent with chromatin-associated localization and genomic surveillance functions. PARP1 expression is broadly observed across proliferating tissues and tumor cell populations due to its essential role in DNA repair and nuclear stress adaptation. Recombinant rabbit monoclonal clone CFD-16 demonstrates a highly specific approach for studying PARP1-associated DNA repair biology, chromatin regulation, and stress signaling pathways. Knockdown validation additionally supports target-specific recognition of PARP1 protein expression across multiple experimental systems.

PARP1 remains one of the most clinically significant DNA repair proteins because of its central role in genomic maintenance and therapeutic response modulation. A selection of phospho-PARP1 antibody products targeting phosphorylation-dependent PARP1 signaling pathways is also available for investigation of DNA damage response activation and chromatin stress signaling mechanisms.

Explore additional chromatin-associated and nuclear-localized proteins in our Nuclear Marker Antibodies collection, featuring targets involved in DNA repair, transcriptional regulation, and genomic stability signaling.

Application Notes

Optimal dilution of the PARP1 Antibody / DNA Repair Enzyme Antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to human PARP (auto-modification/catalytic segment) was used as the immunogen for the PARP antibody.

Storage

Store the PARP antibody at -20oC.

Alternate Names

PARP antibody, Poly ADP-ribose polymerase 1 antibody, ADPRT antibody, PPOL antibody, Nuclear DNA repair protein antibody

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