Parathyroid Hormone Antibody N Terminus [clone SPM604] (V2808)

Description

Parathyroid hormone (PTH), encoded by the PTH gene, is a peptide hormone produced by endocrine chief cells of the parathyroid glands and functions as a central regulator of calcium and phosphate homeostasis. By acting on bone, kidney, and vitamin D metabolism, PTH maintains circulating calcium levels necessary for skeletal health and cellular signaling. Parathyroid Hormone Antibody N Terminus enables detection of Parathyroid hormone while specifically recognizing the amino-terminal portion of the hormone in endocrine tissues and related research samples.

The amino-terminal region of Parathyroid hormone contains the biologically active portion of the peptide responsible for receptor activation and downstream signaling. Antibodies directed against this region are frequently described in the literature as PTH N-terminal antibody, PTH N-terminus antibody, or Parathyroid hormone N-terminal antibody, reflecting recognition of the hormone’s amino-terminal domain. Because the N-terminal portion of PTH contains the sequence responsible for receptor binding and biological activity, antibodies targeting this region are useful tools for studying hormone synthesis, processing, and endocrine signaling mechanisms.

PTH is synthesized in parathyroid chief cells as preproparathyroid hormone and undergoes intracellular processing to generate the mature peptide hormone before secretion. Within tissue sections, Parathyroid hormone is typically localized in the cytoplasm of endocrine chief cells where hormone synthesis and storage occur. Detection of this cytoplasmic staining pattern allows identification of hormone-producing parathyroid cells and supports studies investigating endocrine cell populations and hormone production within parathyroid gland tissue.

PTH antibodies are widely used in endocrine biology and pathology research involving parathyroid tissue and related tumors. Detection of Parathyroid hormone expression assists in confirming parathyroid origin in endocrine lesions and contributes to characterization of parathyroid adenoma, parathyroid hyperplasia, and parathyroid carcinoma. Because parathyroid tissue can sometimes resemble adjacent thyroid structures morphologically, immunodetection using a PTH antibody provides a useful molecular marker for identifying parathyroid-derived endocrine cells.

This mouse monoclonal antibody clone SPM604 recognizes the N-terminal region of Parathyroid hormone. A PTH antibody directed against the N-terminus supports research investigating hormone biosynthesis, endocrine signaling pathways, and tissue expression patterns of Parathyroid hormone in endocrine tissues.

Application Notes

Optimal dilution of the Parathyroid Hormone Antibody N Terminus should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A synthetic peptide corresponding to amino acids 1-34 of human PTH was used as the immunogen for the Parathyroid Hormone antibody.

Storage

Store the Parathyroid Hormone antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

PTH antibody, Parathyroid hormone antibody, PTH N-terminal antibody, Parathyroid hormone N-terminal antibody