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Home >> Antibodies >> Par4 Antibody / Pawr

Par4 Antibody / Pawr (RQ6147)

  Catalog No Formulation Size Price (USD)  
Image RQ6147 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 359
Western blot testing of 1) rat kidney, 2) rat stomach, 3) rat liver, 4) mouse liver, 5) mouse pancreas, 6) mouse testis and 7) mouse HEPA1-6 lysate with Par4 antibody. Predicted molecular weight ~45 kDa.
IHC staining of FFPE mouse brain with Par4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain with Par4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat intestine with Par4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of mouse HEPA1-6 cells with Par4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Par4 antibody.
Immunofluorescent staining of FFPE mouse MFC cells (mouse forestomach carcinoma) with Par4 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Availability 1-3 business days
Species Reactivity Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q925B0
Localization Cytoplasmic, nuclear
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow cytometry : 1-3ug/million cells
Immunofluorescence : 5ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This Par4 antibody is available for research use only.
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Description

PRKC, apoptosis, WT1, regulator, also known as PAWR or Prostate apoptosis response-4 (Par-4), is a human gene. This gene encodes a tumor suppressor protein that selectively induces apoptosis in cancer cells through intracellular and extracellular mechanisms. The intracellular mechanism involves the inhibition of pro-survival pathways and the activation of Fas-mediated apoptosis, while the extracellular mechanism involves the binding of a secreted form of this protein to glucose regulated protein 78 (GRP78) on the cell surface, which leads to activation of the extrinsic apoptotic pathway. This gene is located on the unstable human chromosomal 12q21 region and is often deleted or mutated different tumors. The encoded protein also plays an important role in the progression of age-related diseases.

Application Notes

Optimal dilution of the Par4 antibody should be determined by the researcher.

Immunogen

A mouse recombinant partial protein (amino acids T13-R333) was used as the immunogen for the Par4 antibody.

Storage

After reconstitution, the Par4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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