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Home >> Antibodies >> Pancreas/duodenum homeobox protein 1 Antibody / PDX1

Pancreas/duodenum homeobox protein 1 Antibody / PDX1 [clone PCRP-PDX1-2C11] (V9260)

  Catalog No Formulation Size Price (USD)  
Image V9260-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9260-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9260SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Immunofluorescent staining of PFA-fixed human HeLa cells using Pancreas/duodenum homeobox protein 1 antibody (green, clone PCRP-PDX1-2C11) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with Pancreas/duodenum homeobox protein 1 antibody (blue, clone PCRP-PDX1-2C11), and unstained cells (red).
SDS-PAGE analysis of purified, BSA-free Pancreas/duodenum homeobox protein 1 antibody (clone PCRP-PDX1-2C11) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Pancreas/duodenum homeobox protein 1 antibody (clone PCRP-PDX1-2C11). These results demonstrate the foremost specificity of the PCRP-PDX1-2C11 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2c
Clone Name PCRP-PDX1-2C11
Purity Protein A/G affinity
UniProt P52945
Localization Nucleus
Applications Flow cytometry : 1-2ug/million cells
Immunofluorescence : 1-2ug/ml
Limitations This Pancreas/duodenum homeobox protein 1 antibody is available for research use only.
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Description

Pancreatic duodenal homeobox-1 protein (PDX-1), also designated Insulin promoter factor (IPF1), Insulin upstream factor 1 (IUF1), somatostatin transactivating factor-1 (STF-1) and glucose-sensitive factor (GSF), is a 282 amino acid homeodomain-containing transcription factor present in pancreatic cells. PDX-1 binds to the sequence C(C/T) and can heterodimerize with PBX. PDX-1 is phosphorylated by the SAPK2 pathway under high glucose concentrations. Mutations in the PDX-1 gene can cause maturity-onset diabetes of the young and pancreatic agenesis. The gene which encodes PDX-1 maps to human chromosome 13q12.2.

Application Notes

Optimal dilution of the Pancreas/duodenum homeobox protein 1 antibody should be determined by the researcher.

Immunogen

Recombinant full-length human PDX1 protein was used as the immunogen for the Pancreas/duodenum homeobox protein 1 antibody.

Storage

Aliquot the Pancreas/duodenum homeobox protein 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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