- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Pan Cytokeratin Antibody / Epithelial Differentiation Marker [clone KRTL/1077 + KRTH/1076] (V3051)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V3051-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
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V3051-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
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V3051SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 | |
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V3051IHC-7ML | Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* | 7 ml | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Rat |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | KRTL/1077 + KRTH/1076 |
| Purity | Protein G affinity chromatography |
| UniProt | pan |
| Localization | Cytoplasmic |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT |
| Limitations | This Pan Cytokeratin Antibody / Epithelial Differentiation Marker is available for research use only. |
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Cytokeratins are a diverse family of intermediate filament proteins that play a central role in defining epithelial cell identity and differentiation. These proteins form cytoskeletal networks through pairing of type I acidic and type II basic keratins, creating filament systems that vary depending on epithelial subtype, maturation state, and functional specialization. Changes in cytokeratin expression patterns reflect alterations in epithelial differentiation, making these proteins valuable indicators of epithelial organization and developmental status.
Pan Cytokeratin Antibody / Epithelial Differentiation Marker (clones KRTL/1077 + KRTH/1076) is designed to detect cytokeratin proteins with emphasis on identifying differentiation-associated patterns in immunohistochemistry. This mouse monoclonal antibody cocktail recognizes multiple cytokeratin isoforms, enabling broad epithelial detection while preserving variations in staining intensity and distribution that reflect underlying differentiation states. Pan cytokeratin antibody, also referred to as cytokeratin cocktail antibody or CK pan antibody, is widely used for evaluating epithelial tissues.
In tissue sections, cytokeratin staining highlights epithelial cells with a cytoplasmic pattern corresponding to intermediate filament networks. Differences in staining intensity and distribution across epithelial compartments can reveal variations in differentiation state, allowing interpretation of epithelial maturation and organization. This is particularly evident in stratified epithelia, where basal and suprabasal layers exhibit distinct structural and functional characteristics.
In glandular tissues, cytokeratin staining can reveal organization of epithelial cells within ducts and secretory structures, providing insight into functional specialization. The ability to visualize these patterns supports analysis of epithelial structure and helps distinguish between different epithelial phenotypes within the same tissue.
In tumor samples, cytokeratin expression patterns often correlate with differentiation status. Well-differentiated carcinomas typically retain strong and organized cytokeratin staining, while poorly differentiated tumors may exhibit reduced or heterogeneous staining patterns. These differences provide useful context for evaluating tumor phenotype and can support interpretation of tumor differentiation.
The combination of clones KRTL/1077 and KRTH/1076 provides broad coverage of cytokeratin proteins, ensuring consistent detection across epithelial subtypes while preserving differentiation-related variation. The mouse monoclonal format supports reproducible staining performance and consistent signal intensity across experiments.
Because this antibody provides broad cytokeratin detection, it is not specific to individual keratin isoforms. Instead, it offers a comprehensive view of epithelial differentiation that complements isoform-specific markers used for detailed characterization of epithelial subtypes.
In studies of epithelial biology, the ability to observe variation in cytokeratin staining patterns supports analysis of tissue organization, cellular maturation, and functional specialization. This makes pan cytokeratin detection a valuable tool for examining epithelial heterogeneity in both normal and disease contexts.
Pan Cytokeratin Antibody clones KRTL/1077 + KRTH/1076 therefore provide a valuable tool for analyzing epithelial differentiation, enabling visualization of structural and functional variation within epithelial tissues and supporting interpretation of epithelial organization in diverse biological samples.
This antibody is part of our Pan Cytokeratin Antibody collection, which enables broad epithelial detection across normal and cancer tissues.
Optimal dilution of the Pan Cytokeratin Antibody / Epithelial Differentiation Marker should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
2. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include Keratins 1, 3, 4, 5, 6, 8, 10, 14, 15, 16, and 19.
Recombinant human KRT77 and KRT76 protein were used as the immunogen for the pan Cytokeratin antibody.
Store the pan Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Pan cytokeratin antibody IHC, epithelial differentiation marker antibody, cytokeratin tissue differentiation antibody, CK pan epithelial phenotype marker, cytokeratin maturation marker antibody
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