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Home >> Antibodies >> Pan Cytokeratin Antibody Cocktail

Pan Cytokeratin Antibody Cocktail [clone Cocktail PAN-CK] (V3070)

  Catalog No Formulation Size Price (USD)  
Image V3070-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 499
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V3070-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 219
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V3070SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 499
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V3070IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 499
Immunofluorescent staining of human HeLa cells with pan Cytokeratin antibody cocktail (clone Cocktail PAN-CK, cyan) and NucSpot nuclear stain (red).
Flow cytometry testing of permeabilized human HeLa cells with pan Cytokeratin antibody cocktail (clone Cocktail PAN-CK); Red=isotype control, Blue= pan Cytokeratin antibody cocktail.
IHC staining of FFPE human squamous cell carcinoma with pan Cytokeratin antibody cocktail (clone Cocktail PAN-CK). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name Cocktail PAN-CK
Purity Protein G affinity chromatography
UniProt Q7Z794 , Q01546
Localization Cytoplasmic
Applications Flow cytometry : 0.5-1ug/million cells
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Immunofluorescence : 1-2ug/ml
Limitations This pan Cytokeratin antibody cocktail is available for research use only.
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Description

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, with 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 55kDa (CK7); 52kDa (CK8); 56.5kDa (CK10); 53kDa (CK13); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 46kDa (CK17); 45kDa (CK18) and 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRT-PAN is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It is useful in characterizing the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and shows high sensitivity in the recognition of epithelial cells and carcinomas.

Application Notes

Optimal dilution of the pan Cytokeratin antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Human epidermal keratin was used as the immunogen for the pan Cytokeratin antibody cocktail.

Storage

Store the pan Cytokeratin antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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