- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Pan Cytokeratin Antibody [clone AE-1 + AE-3] (V2330FITC)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V2330FITC-100T | 500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 Tests | 539 |
|
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | FITC Conjugate |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | AE-1 + AE-3 |
| Purity | Protein G affinity chromatography |
| Localization | Cytoplasmic |
| Applications | Flow Cytometry : 5ul per test per one 10^6 cells in 0.1ml or 5ul per 100ul of whole blood Immunofluorescence : 1:50-1:100 |
| Limitations | This Pan Cytokeratin antibody is available for research use only. |
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Pan Cytokeratin antibody FITC conjugate clones AE1 + AE3 combine broad-spectrum cytokeratin detection with direct conjugation to fluorescein isothiocyanate, producing strong green fluorescence. This conjugated format allows rapid and direct identification of epithelial cells without secondary antibodies, making it highly efficient for multicolor fluorescence-based experiments. NSJ Bioreagents supplies Pan Cytokeratin antibody FITC conjugate clones AE1 + AE3 for reliable detection of epithelial markers in immunology, oncology, and developmental biology.
Pan Cytokeratin antibody FITC conjugate clones AE1 + AE3 provide comprehensive coverage of acidic and basic keratins, ensuring broad recognition of epithelial cells in diverse tissues. The FITC conjugation offers bright green signals suitable for flow-based studies, fluorescence microscopy, and imaging assays.
In pathology and oncology, this conjugated antibody is useful for identifying epithelial tumor cells within mixed cell populations, including detection of disseminated carcinoma cells in lymph nodes or bone marrow aspirates. Researchers also use it to evaluate tumor heterogeneity and epithelial-mesenchymal transitions during cancer progression.
In cell biology, Pan Cytokeratin antibody FITC conjugate clones AE1 + AE3 are applied in organoid systems and stem cell differentiation assays, where they reveal epithelial lineage development. The green fluorescence simplifies multiplex studies where epithelial markers need to be combined with other fluorophore-conjugated antibodies.
Technically, the FITC conjugation reduces assay steps by eliminating secondary antibodies, while providing stable and bright fluorescence. This efficiency makes it suitable for high-throughput and multicolor analysis. Alternate names include epithelial cytokeratin antibody FITC, broad-spectrum cytokeratin antibody FITC, and carcinoma marker antibody FITC.
This pan keratin antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3-6, CK8, CK10, CK14-16, and CK19.
Optimal dilution of the Pan Cytokeratin antibody should be determined by the researcher.
Human epidermal keratin was used as the immunogen for this Pan Cytokeratin antibody.
Store the Pan Cytokeratin antibody at 2-8oC, protected from light.
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