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Home >> Antibodies >> Pan-Cadherin Antibody

Pan-Cadherin Antibody [clone Pan-CAD/8019] (V5473)

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  Catalog No Formulation Size Price (USD)  
Image V5473-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5473-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5473SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
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IHC staining of FFPE human colon tissue with Pan-Cadherin antibody (clone Pan-CAD/8019). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human colon tissue with Pan-Cadherin antibody (clone Pan-CAD/8019). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate carcinoma tissue with Pan-Cadherin antibody (clone Pan-CAD/8019). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Pan-Cadherin antibody (clone Pan-CAD/8019) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name Pan-CAD/8019
Purity Protein A/G affinity
UniProt P12830 , P19022 , P22223 , P33151 , P55283
Localization Cell junction
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This Pan-Cadherin antibody is available for research use only.
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Description

Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The most distal of these cadherins is thought to be responsible for binding specificity, transmembrane domains and carboxy terminal intracellular domains. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as ?-catenin, to regulate cadherin function. Members of this family of adhesion proteins include rat cadherin K (and its human homolog, cadherin 6), R-cadherin, B-cadherin, E/P cadherin and cadherin-5.

Application Notes

Optimal dilution of the Pan-Cadherin antibody should be determined by the researcher.

Immunogen

A recombinant fragment from the C-terminal region of human Cadherin proteins was used as the immunogen for the Pan-Cadherin antibody.

Storage

Aliquot the Pan-Cadherin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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