PAK1 Antibody (RQ6571)

IHC staining of FFPE human lung cancer tissue with PAK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human spleen tissue with PAK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liver cancer tissue with PAK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE mouse lymph node tissue with PAK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE mouse brain tissue with PAK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat lymph node tissue with PAK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat brain tissue with PAK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human U-2 OS cells with PAK1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of 1) human HEK293, 2) human SH-SY5Y, 3) human K562, 4) human Jurkat, 5) rat brain, 6) rat PC-12, 7) mouse brain and 8) mouse NIH 3T3 cell lysate with PAK1 antibody. Expected molecular weight: 60-70 kDa.

Flow cytometry testing of human Raji cells with PAK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PAK1 antibody.

Flow cytometry testing of mouse RAW264.7 cells with PAK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PAK1 antibody.