PAI-1 Antibody / Plasminogen activator inhibitor 1 / SERPINE1 [clone ABHG-19] (RQ5107)

Western blot testing of human HepG2 cell lysate with PAI-1/Plasminogen activator inhibitor 1 antibody. Predicted molecular weight ~45 kDa.

Application of PAI-1 / SERPINE1 antibody in western blot. Western blot analysis of PAI-1 / SERPINE1 antibody (clone ABHG-19) was performed using human U251, A549, U2OS, HepG2, A431, and U-87MG whole cell lysates. A prominent immunoreactive band is observed at approximately 45 kDa across multiple lanes, consistent with the predicted molecular weight of Plasminogen activator inhibitor 1. Stronger expression is evident in U251 and U-87MG lysates, with moderate signal in A549, U2OS, HepG2, and A431 cells, reflecting expected cell-type-dependent SERPINE1 expression. The detected band aligns with the predicted molecular weight of approximately 45 kDa under reducing conditions. PAI-1 is a secreted serpin that can exist in active and latent conformations, and minor intensity variation between lanes may reflect differences in expression levels and secretion dynamics rather than size heterogeneity. The blot demonstrates specific detection of endogenous PAI-1 at its predicted molecular weight in human cell lysates.