- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
PAG1 Antibody / Phosphoprotein associated with glycosphingolipid-enriched microdomains 1 (FY13067)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
FY13067 | Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml | 100 ug | 439 |
PAG1 antibody detects Phosphoprotein associated with glycosphingolipid-enriched microdomains 1, a transmembrane adaptor protein that regulates Src family kinase signaling and immune receptor function. The UniProt recommended name is Phosphoprotein associated with glycosphingolipid-enriched microdomains 1 (PAG1). This scaffold protein, also known as Csk-binding protein (CBP), localizes to lipid rafts in the plasma membrane and mediates inhibitory signaling by recruiting the tyrosine kinase CSK to suppress Src family kinases such as LCK and FYN.
Functionally, PAG1 antibody identifies a 432-amino-acid type I transmembrane protein containing multiple tyrosine residues that undergo phosphorylation following receptor activation. Phosphorylated PAG1 binds CSK, leading to phosphorylation and inactivation of Src kinases at their C-terminal regulatory tyrosine. This mechanism provides a critical negative feedback loop that fine-tunes T cell receptor (TCR) and B cell receptor (BCR) signaling, maintaining immune homeostasis.
The PAG1 gene is located on chromosome 8q21.13 and is expressed in hematopoietic cells, neurons, and various epithelial tissues. In T lymphocytes, PAG1 is enriched in membrane microdomains and associates with LAT, LCK, and CD45, modulating activation thresholds and signaling kinetics. Beyond the immune system, PAG1 participates in neuronal development, cell adhesion, and stress response signaling pathways.
Pathologically, dysregulated PAG1 expression contributes to altered immune signaling and oncogenic kinase activation. Downregulation of PAG1 has been observed in certain lymphomas and leukemias, allowing unchecked Src kinase activity. Conversely, overexpression may suppress cell motility and proliferation. Research using PAG1 antibody supports investigations into lipid raft-associated signaling, immune synapse organization, and signal attenuation mechanisms.
PAG1 antibody is suitable for use in western blotting, immunoprecipitation, and immunofluorescence to detect native or phosphorylated PAG1 in cell lysates or tissue samples. It supports studies focused on immune receptor regulation, kinase signaling networks, and cancer biology. NSJ Bioreagents offers validated PAG1 antibody reagents designed for reliable performance in Src signaling and membrane microdomain research.
Structurally, PAG1 contains an extracellular immunoglobulin-like domain, a single transmembrane segment, and a cytoplasmic tail rich in tyrosine motifs. Its lipid raft localization depends on palmitoylation and interaction with glycosphingolipids. This antibody aids in elucidating PAG1s scaffolding functions that link plasma membrane signaling to intracellular kinase regulation.
Optimal dilution of the PAG1 antibody should be determined by the researcher.
A synthetic peptide corresponding to a sequence at the N-terminus of human PAG1 was used as the immunogen for the PAG1 antibody.
After reconstitution, the PAG1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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