p75NTR Antibody DBO-14 / NGF Receptor Monoclonal Antibody [clone DBO-14] (RQ5203)

p75NTR Antibody DBO-14 by WB in Rat C6 and PC-12 Cell Lysates. Western blot analysis of rat C6 glioma (lane 1) and PC-12 pheochromocytoma (lane 2) cell lysates using rabbit monoclonal p75NTR antibody DBO-14. Both lanes exhibit a strong, distinct immunoreactive band at approximately 70-75 kDa, consistent with the expected electrophoretic migration of glycosylated NGF Receptor (CD271). The observed molecular weight falls within the reported 45-75 kDa range associated with different glycosylation states of p75NTR. The bands demonstrate comparable migration in both rat cell lines, with minimal additional immunoreactivity visible. These results support detection of TNFRSF16 in rat-derived neural cell models.

p75NTR Antibody DBO-14 by IHC in Human Glioma. Immunohistochemistry staining of FFPE human glioma tissue using rabbit monoclonal p75NTR antibody DBO-14. Brown immunoreactivity is observed in scattered tumor cells, with predominantly cytoplasmic and peripheral staining surrounding hematoxylin-counterstained nuclei. Staining intensity varies among individual cells, illustrating heterogeneous NGF Receptor (CD271) expression within the glioma specimen. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM citrate buffer, pH 6, for 10-20 minutes, followed by cooling before antibody incubation.