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Home >> Antibodies >> p57Kip2 Antibody

p57Kip2 Antibody [clone KIP2/7187] (V4153)

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  Catalog No Formulation Size Price (USD)  
Image V4153-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4153-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4153SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human placental tissue. Strong nuclear staining observed using KIP2/7187 at 2ug/ml. Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human placental tissue. Strong nuclear staining observed using KIP2/7187 at 2ug/ml. Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human complete mole (CM) tissue with p57Kip2 antibody. Strong nuclear staining observed using KIP2/7187 mAb at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free p57 antibody (clone KIP2/7187) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG, kappa
Clone Name KIP2/7187
Purity Protein A/G affinity
UniProt P49918
Localization Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This p57Kip2 antibody is available for research use only.
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Description

Recognizes a protein of 57kDa, identified as p57Kip2. It shows no cross-reaction with p27Kip1. p57Kip2 is a potent tight-binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. Anti-p57 has been used as an aide in identification of complete hydatidiform mole (CHM) (no nuclear labeling of cytotrophoblasts and stromal cells) from partial hydatidiform mole (PHM) in which both cytotrophoblasts and stromal cells stain. The histological differentiation of complete mole, partial mole, and hydropic spontaneous abortion is problematic. Most complete hydatidiform moles are diploid, whereas most partial moles are triploid. Ploidy studies will identify partial moles, but will not differentiate complete moles from non-molar gestations. Complete moles carry a high risk of persistent disease and choriocarcinoma, while partial moles have a very low risk. In normal placenta, many cytotrophoblast nuclei and stromal cells are labeled with this antibody. Similar findings apply to PHM and hydropic abortus tissues. Intervillous trophoblastic islands (IVTIs) demonstrate nuclear labeling in all three entities and serve as an internal control.

Application Notes

Optimal dilution of the p57Kip2 antibody should be determined by the researcher.

Immunogen

Recombinant full-length human p57Kip2 protein was used as the immunogen for the p57Kip2 antibody.

Storage

Aliquot the p57Kip2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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