- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
p53 Antibody / Stress Response Transcription Factor Antibody [clone TP53/3890R] (V4457)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V4457-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
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V4457-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
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V4457SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Clone Name | TP53/3890R |
| Purity | Protein A/G affinity |
| UniProt | P04637 |
| Localization | Nuclear |
| Applications | Flow Cytometry : 1-2ug/million cells Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT |
| Limitations | This p53 antibody is available for research use only. |
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Tumor protein p53 (TP53) is a nuclear transcription factor that functions as one of the most important stress response transcription factors in mammalian cells. Acting as a master regulator of cellular stress signaling, p53 integrates diverse stress signals and converts them into transcriptional responses that determine whether a cell adapts, arrests growth, or undergoes apoptosis. The p53 Antibody / Stress Response Transcription Factor Antibody detects this central stress response transcription factor, commonly known as p53, which regulates gene expression programs activated by DNA damage, oncogene activation, oxidative stress, hypoxia, and metabolic imbalance.
p53 antibody, also referred to as TP53 antibody or Tumor protein p53 antibody in the literature, targets a protein that serves as a master stress response transcription factor coordinating cellular defense mechanisms. Under normal physiological conditions the stress response transcription factor p53 is maintained at very low levels through continuous ubiquitin-mediated degradation primarily controlled by the E3 ubiquitin ligase MDM2. When cells encounter stress signals such as DNA damage or oncogenic signaling, multiple upstream kinases including ATM, ATR, CHK1, and CHK2 modify p53 and its regulatory partners, preventing degradation and allowing rapid accumulation of the stress response transcription factor in the nucleus.
Once stabilized, the stress response transcription factor p53 binds sequence-specific DNA response elements within promoters of numerous stress-regulated genes. Through this transcriptional activity the stress response transcription factor p53 activates gene networks that control cell cycle arrest, DNA repair, apoptosis, senescence, and metabolic adaptation. One of the best characterized transcriptional targets is CDKN1A (p21), which halts cell cycle progression following DNA damage. Additional transcriptional targets include genes involved in apoptosis such as BAX, PUMA, and NOXA, which eliminate cells that cannot recover from severe stress.
By regulating these stress-responsive transcriptional programs, the stress response transcription factor p53 acts as a molecular hub linking cellular stress detection to protective biological outcomes. Mild stress conditions often trigger p53-dependent cell cycle arrest and repair pathways that restore cellular homeostasis. In contrast, persistent or severe stress signals activate transcriptional programs that promote apoptosis, preventing survival of genetically compromised cells and suppressing tumor formation.
Disruption of stress response transcription factor activity due to TP53 mutation is one of the most common molecular alterations in human cancer. Loss of TP53 signaling prevents cells from mounting appropriate transcriptional responses to stress, allowing survival and proliferation of genetically unstable cells. In many tumor types mutant forms of the stress response transcription factor accumulate within the nucleus due to impaired degradation mechanisms, producing elevated p53 protein levels frequently detected in cancer tissues.
A recombinant rabbit monoclonal p53 antibody such as clone TP53/3890R is suitable for detecting the p53 stress response transcription factor in studies examining cellular stress signaling pathways, transcriptional regulation of stress responses, and molecular mechanisms underlying tumor development.
Optimal dilution of the p53 Antibody / Stress Response Transcription Factor Antibody should be determined by the researcher.
Recombinant human full-length protein was used as the immunogen for the p53 Antibody / Stress Response Transcription Factor Antibody.
Aliquot the p53 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
TP53 antibody, Tumor protein p53 antibody, Cellular tumor antigen p53 antibody, Phosphoprotein p53 antibody, Transformation related protein 53 antibody
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