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Home >> Antibodies >> p53 Antibody / TP53

p53 Antibody / TP53 [clone PAb240] (V8130)

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  Catalog No Formulation Size Price (USD)  
Image V8130-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 559
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V8130-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 259
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V8130SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using p53 antibody (clone PAb240). These results demonstrate the foremost specificity of the PAb240 mAb.Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free p53 antibody (clone PAb240) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name PAb240
Purity Protein G affinity chromatography
UniProt P04637
Localization Nuclear
Applications ELISA (order BSA-free Format For Coating) :
Limitations This p53 antibody is available for research use only.
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Description

p53 antibody recognizes Tumor protein p53, a nuclear phosphoprotein encoded by the human TP53 gene that functions as a central regulator of genomic integrity. p53 antibody detects a stress-responsive transcription factor that becomes activated following DNA damage, oncogene activation, oxidative stress, and other cellular insults. Under normal physiologic conditions, p53 levels remain low due to rapid degradation mediated by MDM2, but cellular stress stabilizes the protein and promotes its accumulation in the nucleus where it regulates transcription of genes controlling cell cycle arrest, apoptosis, and senescence.

p53 antibody, also referred to as TP53 antibody and tumor suppressor p53 antibody in the literature, targets a protein composed of a transactivation domain, a sequence-specific DNA-binding core domain, and an oligomerization domain required for tetramer formation. This tetrameric structure enables p53 to bind promoter regions of genes such as CDKN1A, BAX, and GADD45, coordinating DNA repair and growth arrest responses. Through these mechanisms, p53 serves as a critical checkpoint guardian preventing propagation of damaged DNA.

The TP53 gene is located on chromosome 17p13.1 and represents the most frequently mutated tumor suppressor gene in human cancer. Missense mutations within the DNA-binding domain often result in conformationally altered p53 protein that accumulates in the nucleus due to impaired degradation. Such mutant forms can exhibit dominant-negative effects or gain-of-function properties that contribute to tumor progression.

Clone PAb240 is historically significant because it preferentially recognizes a conformational epitope associated with mutant p53 protein. This makes p53 antibody clone PAb240 particularly useful in research applications investigating mutant p53 accumulation in tumor tissues and experimental models. The ability to distinguish conformationally altered p53 variants has contributed to extensive use of this clone in cancer biology studies.

Elevated nuclear p53 expression is commonly observed in colorectal carcinoma, breast carcinoma, lung carcinoma, ovarian carcinoma, and many additional malignancies. In contrast, normal tissues typically demonstrate minimal p53 staining due to short protein half-life. Detection of nuclear p53 accumulation therefore supports investigations into TP53 mutation status, tumor suppressor pathway disruption, and mechanisms of oncogenic transformation.

An antibody to p53 is suitable for detecting nuclear p53 expression and for studying DNA damage signaling, tumor suppressor pathways, and mutant p53 biology in relevant research applications.

Application Notes

Optimal dilution of the p53 antibody should be determined by the researcher.

Immunogen

Gel-Purified p53-beta-galactosidase fusion protein containing murine p53 from amino acids 14-389 was used as the immunogen for the p53 antibody.

Storage

Store the p53 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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