p53 Antibody Clone DO-1 / TP53 Tumor Suppressor Antibody [clone DO-1] (V8132)

p53 Antibody Clone DO-1 / TP53 Tumor Suppressor Antibody. Western blot analysis of human HeLa cell lysate demonstrates detection of Tumor protein p53 using p53 Antibody Clone DO-1. A band is detected at approximately 53 kDa, consistent with the predicted molecular weight of p53 / TP53.

p53 Antibody Clone DO-1 / TP53 Tumor Suppressor Antibody. Immunohistochemistry analysis of FFPE human colon carcinoma tissue demonstrates strong nuclear staining of tumor epithelial cells using p53 Antibody Clone DO-1. The HRP-DAB brown chromogenic signal highlights nuclear localization of Tumor protein p53 within malignant epithelial cells, while surrounding stromal cells show comparatively weaker staining. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 minutes followed by cooling prior to staining.

p53 Antibody Clone DO-1 / TP53 Tumor Suppressor Antibody. Protein microarray specificity validation using a HuProt(TM) human protein array containing more than 19,000 full-length human proteins demonstrates strong selective binding of p53 Antibody Clone DO-1 to TP53. The ranked signal plot shows TP53 as the dominant target with the highest signal intensity compared with other proteins on the array, supporting the specificity of clone DO-1 for Tumor protein p53.
The Z-score represents the strength of antibody binding to each protein target detected using a fluorescently labeled secondary antibody. Z-scores are expressed in standard deviations above the mean signal across the array. Proteins are ranked by descending Z-score, and the S-score represents the difference between adjacent Z-scores in the ranked list. A high S-score indicates strong specificity of the antibody for its intended target relative to other proteins present on the array.

SDS-PAGE analysis of purified, BSA-free p53 antibody (clone DO-1) as confirmation of integrity and purity.